Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 6H9] (IgG1,k) to Human Ubiquilin 2 / UBQLN2
ICC, Immunofluorescence, Western blot
Human Ubiquilin 2 / UBQLN2
Human, Mouse (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [6H9]
Western blot (1:1000 - 1:2000)
Specificity and Use
Ubiquilin 2 / UBQLN2 antibody was raised against human ubiquilin 2 expressed in and purified from E. coli.
Clone 6H9 is known to react with from human and mouse.
Try at dilutions of ~1:1000 for immunofluorescence. For western blots try at 1:1000-1:2000. A suitable control tissue is HeLa cell lysates. The ubiqulin 2 protein runs at about ~66 kDa (human) and 68 kDa (mouse) on SDS-PAGE gels
Ubiquilin 2 / UBQLN2 is an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases; and thus, are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation.
HeLa cell cultures stained with Ubiquilin 2 / UBQLN2 antibody (green) and chicken polyclonal antibody to vimentin (red). In most individual cells ubiquilin 2 is present diffusely in the cytoplasm of cells, though some cells show enrichment of the protein in spherical autophagosome-like structure.
Western blot analysis of untransfected primary mouse neuron and glia cell cultures (lane 1), the same cells transduced with human ubiquilin 2 wild type (lane 2), with ubiquilin 2 P506T mutant (lane 3), with ubiquilin 2 P497S mutant (lane 4) and with enhanced GFP control (lane 5), all probed with Ubiquilin 2 / UBQLN2 antibody. Also seen is Ubiquilin 2 / UBQLN2 antibody staining in HeLa cells (lane 6) and 3T3 cells (lane 7). In primary mouse neuron and glia cell culture, endogenous ubiquilin 2 appears as a weak band at 68 kDa in all transduced and non-transduced cells, indicating low endogenous expression of mouse ubiquilin 2. Strong bands are seen in the cells transduced with human wild type or mutant ubiquilin 2. Small proteins run at 50 kDa in these cells are the fragments of ubiquilin 2. Note, ubiquilin 2 run at ~66 kDa in human HeLa cells and 68 kDa in rodent 3T3 cells.
Requested From: United States
Date Requested: 12/6/2016