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Anti-Ubiquilin 2 / UBQLN2 Antibody (clone 6H9) LS-C204504

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Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $285
Inquire for larger quantities

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Most Popular Ubiquilin 2 / UBQLN2 Antibodies

Anti-Ubiquilin 2 / UBQLN2 Antibody (clone 6H9) LS-C204504
Mouse Monoclonal [clone 6H9] (IgG1,k) to Human Ubiquilin 2 / UBQLN2
Human, Mouse
ICC, Immunofluorescence, Western blot
Unconjugated
Immunocytochemistry Image
Anti-Ubiquilin 2 / UBQLN2 Antibody (N-Terminus, AP) LS-C283983
Rabbit Polyclonal (IgG) to Human Ubiquilin 2 / UBQLN2
Human
IHC, Western blot, ELISA
AP Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 6H9] (IgG1,k) to Human Ubiquilin 2 / UBQLN2
Human, Mouse
ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human Ubiquilin 2 / UBQLN2
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [6H9]
Unconjugated
Affinity purified
Unmodified

Applications

  • ICC (1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:1000 - 1:2000)

Specificity and Use

Ubiquilin 2 / UBQLN2 antibody was raised against human ubiquilin 2 expressed in and purified from E. coli.
Clone 6H9 is known to react with from human and mouse.
Try at dilutions of ~1:1000 for immunofluorescence. For western blots try at 1:1000-1:2000. A suitable control tissue is HeLa cell lysates. The ubiqulin 2 protein runs at about ~66 kDa (human) and 68 kDa (mouse) on SDS-PAGE gels

Packaging

PBS, 10 mM sodium azide, 50% glycerol.
Store at -20°C.
For research use only.

About Ubiquilin 2 / UBQLN2

Q9UHD9 NM_013444 NP_038472.2

UBQLN2 Antibody, ALS15 Antibody, DSK2 homolog Antibody, CHAP1 Antibody, HPLIC-2 Antibody, LIC-2 Antibody, N4BP4 Antibody, Nedd4 binding protein 4 Antibody, HRIHFB2157 Antibody, PLIC2 Antibody, PLIC-2 Antibody, Ubiquilin-2 Antibody, Ubiquilin 2 Antibody, DSK2 Antibody, RIHFB2157 Antibody

Ubiquilin 2 / UBQLN2 is an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases; and thus, are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation.

Immunocytochemistry

Immunocytochemistry
HeLa cell cultures stained with Ubiquilin 2 / UBQLN2 antibody (green) and chicken polyclonal antibody to vimentin (red). In most individual cells ubiquilin 2 is present diffusely in the cytoplasm of cells, though some cells show enrichment of the protein in spherical autophagosome-like structure.

Western blot

Western blot
Western blot analysis of untransfected primary mouse neuron and glia cell cultures (lane 1), the same cells transduced with human ubiquilin 2 wild type (lane 2), with ubiquilin 2 P506T mutant (lane 3), with ubiquilin 2 P497S mutant (lane 4) and with enhanced GFP control (lane 5), all probed with Ubiquilin 2 / UBQLN2 antibody. Also seen is Ubiquilin 2 / UBQLN2 antibody staining in HeLa cells (lane 6) and 3T3 cells (lane 7). In primary mouse neuron and glia cell culture, endogenous ubiquilin 2 appears as a weak band at 68 kDa in all transduced and non-transduced cells, indicating low endogenous expression of mouse ubiquilin 2. Strong bands are seen in the cells transduced with human wild type or mutant ubiquilin 2. Small proteins run at 50 kDa in these cells are the fragments of ubiquilin 2. Note, ubiquilin 2 run at ~66 kDa in human HeLa cells and 68 kDa in rodent 3T3 cells.

Requested From: United States
Date Requested: 12/6/2016

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