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Catalog Number Size Price
LS-C204504-100 100 µl (1 mg/ml) $376 
Ubiquilin 2 / UBQLN2 Antibody - HeLa cell cultures stained with Ubiquilin 2 / UBQLN2 antibody (green) and chicken polyclonal antibody to vimentin (red). In most individual cells ubiquilin 2 is present diffusely in the cytoplasm of cells, though some cells show enrichment of the protein in spherical autophagosome-like structure.
Ubiquilin 2 / UBQLN2 Antibody - Western blot analysis of untransfected primary mouse neuron and glia cell cultures (lane 1), the same cells transduced with human ubiquilin 2 wild type (lane 2), with ubiquilin 2 P506T mutant (lane 3), with ubiquilin 2 P497S mutant (lane 4) and with enhanced GFP control (lane 5), all probed with Ubiquilin 2 / UBQLN2 antibody. Also seen is Ubiquilin 2 / UBQLN2 antibody staining in HeLa cells (lane 6) and 3T3 cells (lane 7). In primary mouse neuron and glia cell culture, endogenous ubiquilin 2 appears as a weak band at 68 kDa in all transduced and non-transduced cells, indicating low endogenous expression of mouse ubiquilin 2. Strong bands are seen in the cells transduced with human wild type or mutant ubiquilin 2. Small proteins run at 50 kDa in these cells are the fragments of ubiquilin 2. Note, ubiquilin 2 run at ~66 kDa in human HeLa cells and 68 kDa in rodent 3T3 cells.
Ubiquilin 2 / UBQLN2 Antibody - HeLa cell cultures stained with Ubiquilin 2 / UBQLN2 antibody (green) and chicken polyclonal antibody to vimentin (red). In most individual cells ubiquilin 2 is present diffusely in the cytoplasm of cells, though some cells show enrichment of the protein in spherical autophagosome-like structure.
Ubiquilin 2 / UBQLN2 Antibody - Western blot analysis of untransfected primary mouse neuron and glia cell cultures (lane 1), the same cells transduced with human ubiquilin 2 wild type (lane 2), with ubiquilin 2 P506T mutant (lane 3), with ubiquilin 2 P497S mutant (lane 4) and with enhanced GFP control (lane 5), all probed with Ubiquilin 2 / UBQLN2 antibody. Also seen is Ubiquilin 2 / UBQLN2 antibody staining in HeLa cells (lane 6) and 3T3 cells (lane 7). In primary mouse neuron and glia cell culture, endogenous ubiquilin 2 appears as a weak band at 68 kDa in all transduced and non-transduced cells, indicating low endogenous expression of mouse ubiquilin 2. Strong bands are seen in the cells transduced with human wild type or mutant ubiquilin 2. Small proteins run at 50 kDa in these cells are the fragments of ubiquilin 2. Note, ubiquilin 2 run at ~66 kDa in human HeLa cells and 68 kDa in rodent 3T3 cells.
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Monoclonal Mouse anti‑Human Ubiquilin 2 / UBQLN2 Antibody (clone 6H9, IF, WB) LS‑C204504

Monoclonal Mouse anti‑Human Ubiquilin 2 / UBQLN2 Antibody (clone 6H9, IF, WB) LS‑C204504

Antibody:
Ubiquilin 2 / UBQLN2 Mouse anti-Human Monoclonal (6H9) Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$376
LS-C204504-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Ubiquilin 2 / UBQLN2 Mouse anti-Human Monoclonal (6H9) Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
UBQLN2 antibody LS-C204504 is an unconjugated mouse monoclonal antibody to UBQLN2 (Ubiquilin 2) from human. It is reactive with human, mouse and rat. Validated for ICC, IF and WB.
Target
Human Ubiquilin 2 / UBQLN2
Synonyms
UBQLN2 | ALS15 | DSK2 homolog | CHAP1 | HPLIC-2 | LIC-2 | N4BP4 | Nedd4 binding protein 4 | HRIHFB2157 | PLIC2 | PLIC-2 | Ubiquilin-2 | Ubiquilin 2 | DSK2 | RIHFB2157
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
6H9
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Human ubiquilin 2 expressed in and purified from E. coli.
Specificity
Clone 6H9 is known to react with from human and mouse.
Applications
  • ICC (1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:1000 - 1:2000)
Usage
Try at dilutions of ~1:1000 for immunofluorescence. For western blots try at 1:1000-1:2000. A suitable control tissue is HeLa cell lysates. The ubiqulin 2 protein runs at about ~66 kDa (human) and 68 kDa (mouse) on SDS-PAGE gels
Presentation
PBS, 10 mM sodium azide, 50% glycerol.
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Ubiquilin 2 / UBQLN2
Q9UHD9 NM_013444 NP_038472.2

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Requested From: United States
Date Requested: 4/26/2024