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Anti-UBE2N / UBC13 Antibody (aa40-51) IHC-plus™ LS-B433

Note: This antibody replaces LS-C18913


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular UBE2N / UBC13 Antibodies

Anti-UBE2N / UBC13 Antibody (aa40-51) IHC-plus™ LS-B433
Goat Polyclonal to Human UBE2N / UBC13
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Immunohistochemistry Image
Anti-UBE2N / UBC13 Antibody (C-Terminus) IHC-plus™ LS-B1433
Rabbit Polyclonal to Human UBE2N / UBC13
Human, Mouse, Rat
IHC - Paraffin, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human UBE2N / UBC13
IHC - Paraffin, Immunofluorescence, Western blot, ELISA


Human UBE2N / UBC13
Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:25000)

Specificity and Use

UBE2N / UBC13 antibody was raised against synthetic peptide from human UBE2N.
Amino acids 40-51 of human UBC13 protein.
Immunohistochemistry: LS-B433 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B433 was determined to be 2.5 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About UBE2N / UBC13

P61088 NM_003348 NP_003339.1

UBE2N Antibody, BLU Antibody, UbcH13 Antibody, UbcH-ben Antibody, Ubiquitin carrier protein N Antibody, Yeast UBC13 homolog Antibody, UBC13 Antibody, Ubiquitin-protein ligase N Antibody

The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.


Anti-UBE2N antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B433 concentration 2.5 ug/ml.

Western blot

Western blot
Anti-UBC13 Antibody - Western Blot. Western blot of affinity purified anti-UBC13 antibody shows detection of UBC13 protein in human small intestine lysate (lane 1) but not in mouse thymus lysate (lane 2). The heavily stained band in lane 1 (arrowhead) indicates this particular gel was overloaded with protein. The identity of minor reactive bands is unknown but could represent E2 complexes. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:10000 dilution of Alexa FluorTM 680 conjugated Rb-a-Goat IgG. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.

Requested From: United States
Date Requested: 10/24/2016

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