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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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UBE2G1 Antibody (clone 1C12‑1B2) LS‑C198396
UBE2G1 antibody LS-C198396 is an unconjugated mouse monoclonal antibody to human UBE2G1. Validated for ELISA, IF and WB.
Catalog
Size
Price
LS-C198396-100
100 µg
Unavailable

Popular UBE2G1 Products

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse
Applications: IHC, Western blot, ELISA
Immunofluorescence analysis of U20S cells.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Antibody
Species: Human
Applications: Immunofluorescence, Western blot, ELISA

Product Description

UBE2G1 antibody LS-C198396 is an unconjugated mouse monoclonal antibody to human UBE2G1. Validated for ELISA, IF and WB.
About UBE2G1
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family and catalyzes the covalent attachment of ubiquitin to other proteins. P62253 NM_003342 NP_003333.1

UBE2G1 Antibody, UBC7 Antibody, UBE2G Antibody, Ubiquitin carrier protein G1 Antibody, Ubiquitin-protein ligase G1 Antibody, E217K Antibody


Specifications

Target
Human UBE2G1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [1C12-1B2]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Immunofluorescence (20 µg/ml)
  • Western blot
  • ELISA
Immunogen
UBE2G1 antibody was raised against uBE2G1 (AAH02775, aa1-170 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human UBE2G1
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Immunofluorescence of monoclonal antibody to UBE2G1 on HeLa cell. [antibody concentration 20 ug/ml]
Immunofluorescence of monoclonal antibody to UBE2G1 on HeLa cell. [antibody concentration 20 ug/ml]

Western blot

Western Blot analysis of UBE2G1 expression in transfected 293T cell line by UBE2G1 monoclonal antibody (M01), clone 1C12-1B2.Lane 1: UBE2G1 transfected lysate (Predicted MW: 19.5 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of UBE2G1 expression in transfected 293T cell line by UBE2G1 monoclonal antibody (M01), clone 1C12-1B2.Lane 1: UBE2G1 transfected lysate (Predicted MW: 19.5 KDa).Lane 2: Non-transfected lysate.

Immunofluorescence

Immunofluorescence of monoclonal antibody to UBE2G1 on HeLa cell. [antibody concentration 20 ug/ml]
Immunofluorescence of monoclonal antibody to UBE2G1 on HeLa cell. [antibody concentration 20 ug/ml]

Western blot

Western Blot analysis of UBE2G1 expression in transfected 293T cell line by UBE2G1 monoclonal antibody (M01), clone 1C12-1B2.Lane 1: UBE2G1 transfected lysate (Predicted MW: 19.5 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of UBE2G1 expression in transfected 293T cell line by UBE2G1 monoclonal antibody (M01), clone 1C12-1B2.Lane 1: UBE2G1 transfected lysate (Predicted MW: 19.5 KDa).Lane 2: Non-transfected lysate.

Immunofluorescence

Immunofluorescence of monoclonal antibody to UBE2G1 on HeLa cell. [antibody concentration 20 ug/ml]
Immunofluorescence of monoclonal antibody to UBE2G1 on HeLa cell. [antibody concentration 20 ug/ml]

Western blot

Western Blot analysis of UBE2G1 expression in transfected 293T cell line by UBE2G1 monoclonal antibody (M01), clone 1C12-1B2.Lane 1: UBE2G1 transfected lysate (Predicted MW: 19.5 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of UBE2G1 expression in transfected 293T cell line by UBE2G1 monoclonal antibody (M01), clone 1C12-1B2.Lane 1: UBE2G1 transfected lysate (Predicted MW: 19.5 KDa).Lane 2: Non-transfected lysate.

Immunofluorescence

Immunofluorescence of monoclonal antibody to UBE2G1 on HeLa cell. [antibody concentration 20 ug/ml]
Immunofluorescence of monoclonal antibody to UBE2G1 on HeLa cell. [antibody concentration 20 ug/ml]

Western blot

Western Blot analysis of UBE2G1 expression in transfected 293T cell line by UBE2G1 monoclonal antibody (M01), clone 1C12-1B2.Lane 1: UBE2G1 transfected lysate (Predicted MW: 19.5 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of UBE2G1 expression in transfected 293T cell line by UBE2G1 monoclonal antibody (M01), clone 1C12-1B2.Lane 1: UBE2G1 transfected lysate (Predicted MW: 19.5 KDa).Lane 2: Non-transfected lysate.


Requested From: 
Date Requested: 6/20/2018

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