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Anti-UBE2F Antibody LS-C155553

Catalog Size Price
LS-C155553-100 100 µl (1 mg/ml) Unavailable

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100% Guaranteed
Rabbit Polyclonal (IgG) to Human UBE2F
Western blot


Human UBE2F
Human (tested or 100% immunogen sequence identity)
Mouse, Bovine, Chicken (at least 90% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


Western blot (1:500 - 1:3000)

Specificity and Use

UBE2F antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 185 of NCE2 (SwissProt Q969M7). Percent identity by BLAST analysis: Human (100%); Bovine (97%); Mouse (96%); Chicken (92%); Xenopus (88%); Rat (83%).
Human UBE2F
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.


PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.
For research use only.

About UBE2F

Q969M7 NP_542409.1

UBE2F Antibody, NCE2 Antibody, NEDD8 carrier protein UBE2F Antibody, NEDD8 protein ligase UBE2F Antibody, NEDD8-conjugating enzyme 2 Antibody, NEDD8-conjugating enzyme UBE2F Antibody

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

Western blot

Sample (30 ug of whole cell lysate) A: NT2D1 12% SDS PAGE UBE2F antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: NT2D1 12% SDS PAGE UBE2F antibody diluted at 1:1000

Requested From: 
Date Requested: 4/19/2018

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