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Anti-UBE2F Antibody (aa6-19) LS-C139772

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular UBE2F Antibodies

Anti-UBE2F Antibody (C-Terminus) LS-C117234
Rabbit Polyclonal to Human UBE2F
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Chicken, Xenopus, Zebrafish
Western blot
Unconjugated
Western blot Image
Anti-UBE2F Antibody (aa141-154) IHC-plus™ LS-B6482
Goat Polyclonal to Human UBE2F
Human, Monkey, Mouse
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human UBE2F
Human, Monkey, Mouse, Rat, Bat, Hamster, Horse
Western blot, ELISA
Unconjugated

Details

Human UBE2F
Goat
Human, Monkey, Mouse, Rat, Bat, Hamster, Horse (tested or 100% immunogen sequence identity)
Bovine, Dog, Rabbit, Xenopus (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.05 - 0.2 µg/ml)
  • ELISA (1:32000)

Specificity and Use

UBE2F antibody was raised against synthetic peptide SKLKRDDGLKGSRT-C from the N-terminus of human UBE2F / UBE2F (NP_542409.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Bat, Horse (100%); Rat, Panda, Bovine, Dog, Rabbit, Xenopus (93%); Lizard (86%).
LS-E27661 - Lyophilized - 100 µg - $145.00
aa6-19
Human UBE2F / UBE2F.
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 22kD band observed in Rat Pancreas lysates (calculated MW of 21.1kD according to Human NP_542409.1 and 18.6kD according to Rat NP_001008382.1). Recommended concentration: 0.05-0.2 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About UBE2F

Q969M7 NP_542409.1

UBE2F Antibody, NCE2 Antibody, NEDD8 carrier protein UBE2F Antibody, NEDD8 protein ligase UBE2F Antibody, NEDD8-conjugating enzyme 2 Antibody, NEDD8-conjugating enzyme UBE2F Antibody

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

Western blot

Western blot
UBE2F antibody (0.05 ug/ml) staining of Rat Pancreas lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 12/4/2016

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