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Anti-UBE2D3 / UBCH5C Antibody (N-Terminus) LS-C101997

Ordering

Wt. Vol. Conc. Price
50 µg - - $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular UBE2D3 / UBCH5C Antibodies

Anti-UBE2D3 / UBCH5C Antibody (aa109-136, AP) LS-C272867
Rabbit Polyclonal (IgG) to Human UBE2D3 / UBCH5C
Human
Western blot, ELISA
AP Conjugated
Anti-UBE2D3 / UBCH5C Antibody (aa109-136, APC) LS-C272868
Rabbit Polyclonal (IgG) to Human UBE2D3 / UBCH5C
Human
Western blot, ELISA
APC Conjugated
Anti-UBE2D3 / UBCH5C Antibody (aa109-136, Azide-free, HRP) LS-C272871
Rabbit Polyclonal (IgG) to Human UBE2D3 / UBCH5C
Human
Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human UBE2D3 / UBCH5C
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Chicken
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human UBE2D3 / UBCH5C
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Chicken (tested or 100% immunogen sequence identity)
Bat, Pig, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

UBE2D3 / UBCH5C antibody was raised against synthetic peptide from N-Terminus of human UBE2D3 (P61077, NP_003331). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%); Gibbon, Bat, Pig, Lizard, Xenopus, Trout, Salmon, Smelt, Stickleback, Pike, Zebrafish (92%); Drosophila, Beetle (85%); Goat, Panda, Rabbit, Catfish, Nematode (84%).
LS-E15827 - Lyophilized - 100 µg - $145.00
N-Terminus
Human UBE2D3
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About UBE2D3 / UBCH5C

P61077 NM_003340 NP_003331.1

UBE2D3 Antibody, E2(17)KB3 Antibody, E2(17)KB 3 Antibody, Ubiquitin-protein ligase D3 Antibody, UBCH5C Antibody, UBC4/5 Antibody, UBC5C Antibody, Ubiquitin carrier protein D3 Antibody

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin.

Western blot

Western blot
UBE2D3 antibody LS-C101997 Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/2/2016

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