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UBCH / UBE2H Antibody (clone 3C4‑1A2) LS‑C198397
UBE2H antibody LS-C198397 is an unconjugated mouse monoclonal antibody to human UBE2H (UBCH). Validated for ELISA, IF and WB.
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Species:
Human, Mouse, Rat
Applications:
IHC, Immunofluorescence, Western blot
Species:
Human
Applications:
Western blot, Immunoprecipitation, ELISA
Product Description
UBE2H antibody LS-C198397 is an unconjugated mouse monoclonal antibody to human UBE2H (UBCH). Validated for ELISA, IF and WB.
About UBCH / UBE2H
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family.
P62256
NM_003344
NP_003335.1
UBE2H Antibody, GID3 Antibody, UBC8 Antibody, Ubiquitin carrier protein H Antibody, UBCH Antibody, Ubiquitin-protein ligase H Antibody, E2-20K Antibody, UBCH2 Antibody
Specifications
Target
Human UBCH / UBE2H
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
[3C4-1A2]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
- Immunofluorescence (10 µg/ml)
- Western blot
- ELISA
Immunogen
UBCH / UBE2H antibody was raised against uBE2H (AAH06277, aa1-183 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human UBCH / UBE2H
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Customer Reviews (0)
Images
Immunofluorescence
![Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com/image2/ubch-ube2h-antibody-clone-3c4-1a2-ls-c198397/146890_1435546.jpg)
Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10 ug/ml]
Western blot
Western Blot analysis of UBE2H expression in transfected 293T cell line by UBE2H monoclonal antibody (M01), clone 3C4-1A2.Lane 1: UBE2H transfected lysate(20.7 KDa).Lane 2: Non-transfected lysate.
Western blot
UBE2H monoclonal antibody (M01), clone 3C4-1A2 Western Blot analysis of UBE2H expression in HeLa.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged UBE2H is approximately 0.03 ng/ml as a capture antibody.
Immunofluorescence
![Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com/image2/ubch-ube2h-antibody-clone-3c4-1a2-ls-c198397/146890_3018150.jpg)
Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10 ug/ml]
Western blot
Western Blot analysis of UBE2H expression in transfected 293T cell line by UBE2H monoclonal antibody (M01), clone 3C4-1A2.Lane 1: UBE2H transfected lysate(20.7 KDa).Lane 2: Non-transfected lysate.
Western blot
UBE2H monoclonal antibody (M01), clone 3C4-1A2 Western Blot analysis of UBE2H expression in HeLa.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged UBE2H is approximately 0.03 ng/ml as a capture antibody.
Immunofluorescence
![Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com/image2/ubch-ube2h-antibody-clone-3c4-1a2-ls-c198397/146890_19356.jpg)
Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10 ug/ml]
Western blot
Western Blot analysis of UBE2H expression in transfected 293T cell line by UBE2H monoclonal antibody (M01), clone 3C4-1A2.Lane 1: UBE2H transfected lysate(20.7 KDa).Lane 2: Non-transfected lysate.
Western blot
UBE2H monoclonal antibody (M01), clone 3C4-1A2 Western Blot analysis of UBE2H expression in HeLa.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged UBE2H is approximately 0.03 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10 ug/ml]
Western blot
Western Blot analysis of UBE2H expression in transfected 293T cell line by UBE2H monoclonal antibody (M01), clone 3C4-1A2.Lane 1: UBE2H transfected lysate(20.7 KDa).Lane 2: Non-transfected lysate.
Western blot
UBE2H monoclonal antibody (M01), clone 3C4-1A2 Western Blot analysis of UBE2H expression in HeLa.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged UBE2H is approximately 0.03 ng/ml as a capture antibody.
Requested From:
Date Requested: 6/21/2018
Date Requested: 6/21/2018
