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Anti-UBCH / UBE2H Antibody (clone 3C4-1A2) LS-C198397

Catalog	Size	Price
LS-C198397-100	100 µg	Unavailable

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18 UBCH / UBE2H Antibodies

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Antibody:	Mouse Monoclonal [clone 3C4-1A2] (IgG2a,k) to Human UBCH / UBE2H
Reactivity:	Human
Application:	Immunofluorescence, Western blot, ELISA
Format:	Unconjugated

Anti-UBCH / UBE2H Antibody (aa154-183) LS-C326132

Antibody:	Rabbit Polyclonal (IgG) to Human UBCH / UBE2H
Reactivity:	Human
Application:	Western blot, ELISA
Format:	Unconjugated

Anti-UBCH / UBE2H Antibody (aa41-70) LS-C326133

Antibody:	Rabbit Polyclonal (IgG) to Human UBCH / UBE2H
Reactivity:	Human
Application:	Western blot, ELISA
Format:	Unconjugated

Anti-UBCH / UBE2H Antibody LS-C349070

Antibody:	Rabbit Polyclonal (IgG) to Human UBCH / UBE2H
Reactivity:	Human, Mouse, Rat
Application:	IHC, Immunofluorescence, Western blot
Format:	Unconjugated

Anti-UBCH / UBE2H Antibody LS-C412864

Antibody:	Rabbit Polyclonal to Human UBCH / UBE2H
Reactivity:	Human, Mouse, Rat
Application:	IHC, Western blot
Format:	Unconjugated

Antibody:	Mouse Monoclonal [clone 3C4-1A2] (IgG2a,k) to Human UBCH / UBE2H
Reactivity:	Human
Application:	Immunofluorescence, Western blot, ELISA
Format:	Unconjugated

Details

Target:	Human UBCH / UBE2H
Host:	Mouse
Reactivity:	Human (tested or 100% immunogen sequence identity)
Clonality:	IgG2a,k Monoclonal [3C4-1A2]
Conjugations:	Unconjugated
Purification:	Purified from ascites by Protein A
Modifications:	Unmodified

Applications

Approved:

Immunofluorescence (10 µg/ml)
Western blot
ELISA

Specificity and Use

Immunogen:	UBCH / UBE2H antibody was raised against uBE2H (AAH06277, aa1-183 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity:	Human UBCH / UBE2H
Usage:	Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

Presentation:	PBS, pH 7.2
Storage:	Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Restrictions:	For research use only.

About UBCH / UBE2H

Uniprot: P62256 NCBI: NM_003344 NP_003335.1

Synonyms: UBE2H Antibody, GID3 Antibody, UBC8 Antibody, Ubiquitin carrier protein H Antibody, UBCH Antibody, Ubiquitin-protein ligase H Antibody, E2-20K Antibody, UBCH2 Antibody

Description: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family.

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Immunofluorescence

Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of UBE2H expression in transfected 293T cell line by UBE2H monoclonal antibody (M01), clone 3C4-1A2.Lane 1: UBE2H transfected lysate(20.7 KDa).Lane 2: Non-transfected lysate.

Western blot

UBE2H monoclonal antibody (M01), clone 3C4-1A2 Western Blot analysis of UBE2H expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged UBE2H is approximately 0.03 ng/ml as a capture antibody.

Requested From:
Date Requested: 8/21/2017

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