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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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UBCH / UBE2H Antibody (clone 3C4‑1A2) LS‑C198397 Best Seller
UBE2H antibody LS-C198397 is an unconjugated mouse monoclonal antibody to human UBE2H (UBCH). Validated for ELISA, IF and WB.
Catalog
Size
Price
LS-C198397-100
100 µg
Unavailable

Popular UBCH / UBE2H Products

Immunofluorescence analysis of A549 cells.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
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Species: Human, Mouse, Rat
Applications: IHC, Western blot
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Species: Human
Applications: Western blot, Immunoprecipitation, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

UBE2H antibody LS-C198397 is an unconjugated mouse monoclonal antibody to human UBE2H (UBCH). Validated for ELISA, IF and WB.
About UBCH / UBE2H
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. P62256 NM_003344 NP_003335.1

UBE2H Antibody, GID3 Antibody, UBC8 Antibody, Ubiquitin carrier protein H Antibody, UBCH Antibody, Ubiquitin-protein ligase H Antibody, E2-20K Antibody, UBCH2 Antibody


Specifications

Target
Human UBCH / UBE2H
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [3C4-1A2]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
Immunogen
UBCH / UBE2H antibody was raised against uBE2H (AAH06277, aa1-183 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human UBCH / UBE2H
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunofluorescence

Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of UBE2H expression in transfected 293T cell line by UBE2H monoclonal antibody (M01), clone 3C4-1A2.Lane 1: UBE2H transfected lysate(20.7 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of UBE2H expression in transfected 293T cell line by UBE2H monoclonal antibody (M01), clone 3C4-1A2.Lane 1: UBE2H transfected lysate(20.7 KDa).Lane 2: Non-transfected lysate.

Western blot

UBE2H monoclonal antibody (M01), clone 3C4-1A2 Western Blot analysis of UBE2H expression in HeLa.
UBE2H monoclonal antibody (M01), clone 3C4-1A2 Western Blot analysis of UBE2H expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged UBE2H is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged UBE2H is approximately 0.03 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of UBE2H expression in transfected 293T cell line by UBE2H monoclonal antibody (M01), clone 3C4-1A2.Lane 1: UBE2H transfected lysate(20.7 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of UBE2H expression in transfected 293T cell line by UBE2H monoclonal antibody (M01), clone 3C4-1A2.Lane 1: UBE2H transfected lysate(20.7 KDa).Lane 2: Non-transfected lysate.

Western blot

UBE2H monoclonal antibody (M01), clone 3C4-1A2 Western Blot analysis of UBE2H expression in HeLa.
UBE2H monoclonal antibody (M01), clone 3C4-1A2 Western Blot analysis of UBE2H expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged UBE2H is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged UBE2H is approximately 0.03 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of UBE2H expression in transfected 293T cell line by UBE2H monoclonal antibody (M01), clone 3C4-1A2.Lane 1: UBE2H transfected lysate(20.7 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of UBE2H expression in transfected 293T cell line by UBE2H monoclonal antibody (M01), clone 3C4-1A2.Lane 1: UBE2H transfected lysate(20.7 KDa).Lane 2: Non-transfected lysate.

Western blot

UBE2H monoclonal antibody (M01), clone 3C4-1A2 Western Blot analysis of UBE2H expression in HeLa.
UBE2H monoclonal antibody (M01), clone 3C4-1A2 Western Blot analysis of UBE2H expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged UBE2H is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged UBE2H is approximately 0.03 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of UBE2H expression in transfected 293T cell line by UBE2H monoclonal antibody (M01), clone 3C4-1A2.Lane 1: UBE2H transfected lysate(20.7 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of UBE2H expression in transfected 293T cell line by UBE2H monoclonal antibody (M01), clone 3C4-1A2.Lane 1: UBE2H transfected lysate(20.7 KDa).Lane 2: Non-transfected lysate.

Western blot

UBE2H monoclonal antibody (M01), clone 3C4-1A2 Western Blot analysis of UBE2H expression in HeLa.
UBE2H monoclonal antibody (M01), clone 3C4-1A2 Western Blot analysis of UBE2H expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged UBE2H is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged UBE2H is approximately 0.03 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/21/2018

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