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Anti-UBA1 / UBE1 Antibody LS-C18912

Ordering

Wt. Vol. Conc. Price
500 µg 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular UBA1 / UBE1 Antibodies

Anti-UBA1 / UBE1 Antibody (aa1-30, AP) LS-C272825
Rabbit Polyclonal (IgG) to Human UBA1 / UBE1
Human
IHC, Western blot, ELISA
AP Conjugated
Anti-UBA1 / UBE1 Antibody (aa800-850) IHC-plus™ LS-B14423
Rabbit Polyclonal (IgG) to Human UBA1 / UBE1
Human, Mouse, Rat, Guinea pig, Rabbit
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human UBA1 / UBE1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human UBA1 / UBE1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Unmodified

Applications

  • IHC - Paraffin (2 - 20 µg/ml)
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:2000 - 1:10000)

Specificity and Use

UBA1 / UBE1 antibody was raised against recombinant human UBA1 / UBE1.
Full length Human Ubiquitin Activating Enzyme E1 fused with GST.
This purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~118 kD in size corresponding to UBE1 by western blotting in the appropriate cell lysate or extract.

Packaging

Lyophilized.
100 µl After adding water, will consist of 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide
Store vial at 4 C prior to restoration. Reconstitute with 0.1 ml of deionized water or equivalent. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About UBA1 / UBE1

P22314 NM_003334 NP_003325.2

UBA1 Antibody, A1S9 Antibody, A1ST Antibody, A1S9T Antibody, AMCX1 Antibody, GXP1 Antibody, Protein A1S9 Antibody, SMAX2 Antibody, Ubiquitin-activating enzyme E1 Antibody, UBE1X Antibody, POC20 Antibody, UBE1 Antibody, UBA1A Antibody

Ubiquitin Activating Enzyme (E1), also known as A1S9 and UBE1, is responsible for the first step in ubiquitin-protein isopeptide bond formation. E1 catalyzes the activation of the Cterminal carboxyl group of ubiquitin by forming a high-energy thioester bond in an ATP-dependent manner.

Immunohistochemistry

Immunohistochemistry
Anti-Ubiquitin Activating Enzyme Antibody - Immunohistochemistry. Affinity Purified anti-Ubiquitin Activating Enzyme antibody was used at a 10 ug/ml to detect UBE1 in a variety of tissues including adrenal, breast, colon (epithelium), kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (islet and exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis, thymus, tonsil, and uterus (glandular, stroma). In many cells a punctate nuclear staining was observed. Other cells showed both cytoplasmic and nuclear staining. This image shows UBE1 staining of human lung tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.

Western blot

Western blot
Anti-Ubiquitin Activating Enzyme (E1) Antibody - Western Blot. Western blot of purified anti-Ubiquitin Activating Enzyme (E1) antibody shows detection of a band at ~118 kD corres-ponding to UBE1 (lane 1 800 nm channel). Approximately 35 ug of an A431 whole cell lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX10 ( for 45 min at room temperature. Molecular weight markers are shown in lane 2 (700 nm channel). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 12/3/2016

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