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U2AF1L4 Antibody (aa82‑94) LS‑C87227
U2AF1L4 antibody LS-C87227 is an unconjugated goat polyclonal antibody to human U2AF1L4. Validated for Peptide-ELISA and WB.
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Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Applications:
Western blot
Product Description
U2AF1L4 antibody LS-C87227 is an unconjugated goat polyclonal antibody to human U2AF1L4. Validated for Peptide-ELISA and WB.
About U2AF1L4
RNA-binding protein that function as a pre-mRNA splicing factor. Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Acts by enhancing the binding of U2AF2 to weak pyrimidine tracts. Also participates in the regulation of alternative pre-mRNA splicing. Activates exon 5 skipping of PTPRC during T-cell activation; an event reversed by GFI1.
NP_659424.2
U2AF1L4 Antibody, U2AF1L3V1 Antibody, U2AF1RS3 Antibody, U2 auxiliary factor 26 Antibody, U2AF1-like protein 3 Antibody, U2af26 Antibody, U2AF1-RS3 Antibody, U2AF1L3 Antibody
Specifications
Target
Human U2AF1L4
Host
Goat
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000) & (0.1 - 0.3 µg/ml)
Failed Applications
- IHC - Paraffin
Immunogen
U2AF1L4 antibody was raised against synthetic peptide C-EDGERAVAELSNR from an internal region of human U2AF1L4 (NP_001035515.1 ; NP_659424.2). Percent identity with other species by BLAST analysis: Human (100%), Gibbon (92%), Mouse (85%), Rat (85%), Panda (85%), Dog (85%), Horse (85%), Rabbit (85%), Pig (85%).
Blocking Peptide
LS-E26518 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C87227. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-C87227. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa82-94
Specificity
Human U2AF1L4. This antibody is expected to recognise both reported isoforms (NP_001035515.1 and NP_659424.2). No cross-reactivity with U2AF1 expected.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx. 20Da band observed in Human Brain (Cerebellum) and Lung lysates (calculated MW of 21.3kD according to NP_001035515.1). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Western blot
Antibody (0.1 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Western blot
Antibody (0.1 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Western blot
Antibody (0.1 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Western blot
Antibody (0.1 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Requested From:
Date Requested: 6/22/2018
Date Requested: 6/22/2018
