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Anti-U2AF1L4 Antibody (aa82-94) LS-C87227

Catalog	Size	Price
LS-C87227-100	100 µg (0.5 mg/ml)	Unavailable

Related Products

8 U2AF1L4 Antibodies

Most Popular U2AF1L4 Antibodies

Anti-U2AF1L4 Antibody (aa23-72) LS-C201383

Antibody:	Rabbit Polyclonal to Human U2AF1L4
Reactivity:	Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Application:	Western blot
Format:	Unconjugated

Western blot of Human HepG2. U2AF1L4 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Anti-U2AF1L4 Antibody LS-C346252

Antibody:	Rabbit Polyclonal (IgG) to Human U2AF1L4
Reactivity:	Human
Application:	IHC, Immunofluorescence, Western blot
Format:	Unconjugated

Immunofluorescence analysis of A549 cells.

Anti-U2AF1L4 Antibody LS-C412762

Antibody:	Rabbit Polyclonal to Human U2AF1L4
Reactivity:	Human
Application:	IHC, Immunofluorescence, Western blot
Format:	Unconjugated

Anti-U2AF1L4 Antibody LS-C676666

Antibody:	Rabbit Polyclonal (IgG) to Human U2AF1L4
Reactivity:	Human
Application:	IHC, ELISA
Format:	Unconjugated

Immunohistochemistry of paraffin-embedded human testis tissue at dilution 1:100

Anti-U2AF1L4 Antibody LS-C676669

Antibody:	Rabbit Polyclonal (IgG) to Human U2AF1L4
Reactivity:	Human
Application:	IHC, ELISA
Format:	Unconjugated

Antibody:	Goat Polyclonal to Human U2AF1L4
Reactivity:	Human
Application:	Western blot, ELISA
Format:	Unconjugated

Antibody (0.1 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Details

Target:	Human U2AF1L4
Host:	Goat
Reactivity:	Human (tested or 100% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (0.1 - 0.3 µg/ml)
ELISA (1:32000) & (0.1 - 0.3 µg/ml)

Failed:

IHC - Paraffin

Specificity and Use

Immunogen:

U2AF1L4 antibody was raised against synthetic peptide C-EDGERAVAELSNR from an internal region of human U2AF1L4 (NP_001035515.1 ; NP_659424.2). Percent identity with other species by BLAST analysis: Human (100%), Gibbon (92%), Mouse (85%), Rat (85%), Panda (85%), Dog (85%), Horse (85%), Rabbit (85%), Pig (85%).

Blocking Peptide:

LS-E26518 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C87227. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

aa82-94

Specificity:

Human U2AF1L4. This antibody is expected to recognise both reported isoforms (NP_001035515.1 and NP_659424.2). No cross-reactivity with U2AF1 expected.

Usage:

Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx. 20Da band observed in Human Brain (Cerebellum) and Lung lysates (calculated MW of 21.3kD according to NP_001035515.1). Recommended concentration: 0.1-0.3 ug/ml.

Packaging

Presentation:	Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage:	Store at -20°C. Avoid freeze-thaw cycles.
Restrictions:	For research use only.

About U2AF1L4

NCBI: NP_659424.2

Synonyms: U2AF1L4 Antibody, U2AF1L3V1 Antibody, U2AF1RS3 Antibody, U2 auxiliary factor 26 Antibody, U2AF1-like protein 3 Antibody, U2af26 Antibody, U2AF1-RS3 Antibody, U2AF1L3 Antibody

Description: RNA-binding protein that function as a pre-mRNA splicing factor. Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Acts by enhancing the binding of U2AF2 to weak pyrimidine tracts. Also participates in the regulation of alternative pre-mRNA splicing. Activates exon 5 skipping of PTPRC during T-cell activation; an event reversed by GFI1.

(More About U2AF1L4)

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Western blot

Antibody (0.1 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Requested From:
Date Requested: 4/21/2018

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