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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TUBB / Beta Tubulin Antibody LS‑C155339
Beta Tubulin antibody LS-C155339 is an unconjugated rabbit polyclonal antibody to Beta Tubulin (TUBB) from human and mouse. Validated for ICC, IF, IHC and WB.
Catalog
Size
Price
LS-C155339-100
100 µl (1 mg/ml)
Unavailable

Popular TUBB / Beta Tubulin Products

Antibody
Species: C. elegans, Mammal
Applications: ICC, Western blot
Anti-TUBB / Tubulin, Beta antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1867 concentration 5 ug/ml.
Species: Pig, Human, All species
Applications: IHC, IHC - Paraffin, ICC, Western blot, Inhibition
Anti-TUBB / Tubulin, Beta antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2189 dilution 1:100. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence
Anti-TUBB / Tubulin, Beta antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3442 concentration 10 ug/ml.
Species: Human, Mouse, Rat, Bovine, Rabbit, Chicken, Frog
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Western blot Image
Species: Human, Mouse, Rat
Applications: Western blot, ELISA

Product Description

Beta Tubulin antibody LS-C155339 is an unconjugated rabbit polyclonal antibody to Beta Tubulin (TUBB) from human and mouse. Validated for ICC, IF, IHC and WB.
About TUBB / Beta Tubulin
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. P07437 NM_178014 NP_821133.1

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Specifications

Target
Human TUBB / Beta Tubulin
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Immunofluorescence (1:100 - 1:1000)
  • Western blot (1:1000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 160 and 447 of beta Tubulin. Percent identity by BLAST analysis:
Specificity
Human, mouse TUBB / Beta Tubulin
Usage
Suggested starting dilutions are as follows: Western blot: 1:1000-1:10000, IHC-Paraffin: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Presentation
PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
Storage
Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded HeLa xenograft, using beta Tubulin antibody at 1:500 dilution.
IHC of paraffin-embedded HeLa xenograft, using beta Tubulin antibody at 1:500 dilution.

Immunofluorescence

Immunofluorescence of methanol-fixed HeLa, using beta Tubulin antibody at 1:500 dilution.
Immunofluorescence of methanol-fixed HeLa, using beta Tubulin antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 D: C2C12 E: Raw264.7 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 D: C2C12 E: Raw264.7 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:1000

Western blot

Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:1000

Western blot

Sample (whole cell lysate) A: 293T 20 ug B: 293T 10 ug C: 293T 5 ug 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:10000
Sample (whole cell lysate) A: 293T 20 ug B: 293T 10 ug C: 293T 5 ug 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:10000

Immunohistochemistry

IHC of paraffin-embedded HeLa xenograft, using beta Tubulin antibody at 1:500 dilution.
IHC of paraffin-embedded HeLa xenograft, using beta Tubulin antibody at 1:500 dilution.

Immunofluorescence

Immunofluorescence of methanol-fixed HeLa, using beta Tubulin antibody at 1:500 dilution.
Immunofluorescence of methanol-fixed HeLa, using beta Tubulin antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 D: C2C12 E: Raw264.7 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 D: C2C12 E: Raw264.7 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:1000

Western blot

Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:1000

Western blot

Sample (whole cell lysate) A: 293T 20 ug B: 293T 10 ug C: 293T 5 ug 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:10000
Sample (whole cell lysate) A: 293T 20 ug B: 293T 10 ug C: 293T 5 ug 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:10000

Immunohistochemistry

IHC of paraffin-embedded HeLa xenograft, using beta Tubulin antibody at 1:500 dilution.
IHC of paraffin-embedded HeLa xenograft, using beta Tubulin antibody at 1:500 dilution.

Immunofluorescence

Immunofluorescence of methanol-fixed HeLa, using beta Tubulin antibody at 1:500 dilution.
Immunofluorescence of methanol-fixed HeLa, using beta Tubulin antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 D: C2C12 E: Raw264.7 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 D: C2C12 E: Raw264.7 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:1000

Western blot

Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:1000

Western blot

Sample (whole cell lysate) A: 293T 20 ug B: 293T 10 ug C: 293T 5 ug 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:10000
Sample (whole cell lysate) A: 293T 20 ug B: 293T 10 ug C: 293T 5 ug 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:10000

Immunohistochemistry

IHC of paraffin-embedded HeLa xenograft, using beta Tubulin antibody at 1:500 dilution.
IHC of paraffin-embedded HeLa xenograft, using beta Tubulin antibody at 1:500 dilution.

Immunofluorescence

Immunofluorescence of methanol-fixed HeLa, using beta Tubulin antibody at 1:500 dilution.
Immunofluorescence of methanol-fixed HeLa, using beta Tubulin antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 D: C2C12 E: Raw264.7 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 D: C2C12 E: Raw264.7 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:1000

Western blot

Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:1000

Western blot

Sample (whole cell lysate) A: 293T 20 ug B: 293T 10 ug C: 293T 5 ug 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:10000
Sample (whole cell lysate) A: 293T 20 ug B: 293T 10 ug C: 293T 5 ug 10% SDS PAGE TUBB / Beta Tubulin antibody diluted at 1:10000


Requested From: 
Date Requested: 6/23/2018

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