Target
Human TUBA1B / Tubulin Alpha 1B
Synonyms
TUBA1B | Alpha-tubulin ubiquitous | Alpha tubulin | K-ALPHA-1 | Tubulin, alpha 1b | Tubulin alpha-ubiquitous chain | Tubulin, alpha, ubiquitous | Tubulin K-alpha-1 | Tubulin alpha chain | Tubulin alpha-1B chain
Reactivity
Human, Mouse, Rat, Bovine, Chicken
(tested or 100% immunogen sequence identity)
Predicted
Fish, Algae, Avian, Bacteria, Mammal, Protozoa (at least 90% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.
Specificity
A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Immunofluorescence (0.1 µg/ml)
- Western blot (1:1000)
- ELISA (1:300000)
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Usage
Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kD in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TUBA1B / Tubulin Alpha 1B