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Anti-TSG101 Antibody (clone 5B7) IHC-plus™ LS-B5547

Note: This antibody replaces LS-C133496, LS-C122504

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TSG101 Antibodies

Anti-TSG101 Antibody (C-Terminus) LS-C30930
Rabbit Polyclonal (IgG) to Human TSG101
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Western blot
Unconjugated
Western blot Image
Anti-TSG101 Antibody (clone 5B7) IHC-plus™ LS-B5547
Mouse Monoclonal [clone 5B7] (IgG2a,k) to Human TSG101
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-TSG101 Antibody (N-Terminus, FITC) LS-C220955
Rabbit Polyclonal (IgG) to Human TSG101
Human, Mouse
IHC, Western blot, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 5B7] (IgG2a,k) to Human TSG101
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human TSG101
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [5B7]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA

Specificity and Use

TSG101 antibody was raised against tSG101 (NP_006283, 201 a.a. ~ 281 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human TSG101
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell lysates, cell line lysates and the recombinant protein used as the immunogen.

Packaging

PBS, pH 7.4. Sourced in Ascites.
Long term: -20°C; Short term: -20°C
For research use only.

About TSG101

Q99816 NM_006292 NP_006283.1

TSG101 Antibody, ESCRT-I complex subunit TSG101 Antibody, Tumor susceptibility gene 10 Antibody, Tumor susceptibility protein Antibody, VPS23 Antibody, Tumor susceptibility gene 101 Antibody, TSG10 Antibody

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator.

Immunohistochemistry

Immunohistochemistry
Anti-TSG101 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5547 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to TSG101 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot
TSG101 monoclonal antibody clone 5B7 Western blot of TSG101 expression in K-562.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged TSG101 is approximately 0.03 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 12/4/2016

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