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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TRPM5 Antibody (aa247‑296) LS‑C80116 Best Seller
TRPM5 antibody LS-C80116 is an unconjugated rabbit polyclonal antibody to TRPM5 from human, mouse, rat and other species. Validated for IHC and WB.
Catalog
Size
Price
LS-C80116-100
100 µg (1 mg/ml)
Unavailable

Popular TRPM5 Products

Anti-TRPM5 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6792 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Guinea pig
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry of Human gastric cancer using TRPM5 Polyclonal Antibody at dilution of 1:60.
Species: Human, Mouse
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TRPM5 was detected in paraffin-embedded sections of rat testis tissues using rabbit anti- TRPM5 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
Species: Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Sheep, Chicken
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry - Paraffin Image
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot

Product Description

TRPM5 antibody LS-C80116 is an unconjugated rabbit polyclonal antibody to TRPM5 from human, mouse, rat and other species. Validated for IHC and WB.
About TRPM5
Voltage-modulated Ca2+-activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. Monovalent-specific, non-selective cation channel that mediates the transport of Na+, K+ and Cs+ ions equally well. Activated directly by increases in intracellular Ca2+, but is impermeable to it. Q9NZQ8 NM_014555 NP_055370.1

TRPM5 Antibody, LTRPC5 Antibody, MLSN1 and TRP-related Antibody, MTr1 Antibody, LTrpC-5 Antibody


Specifications

Target
Human TRPM5
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Guinea pig (tested or 100% immunogen sequence identity)
Predicted
Monkey, Dog, Horse (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, Biotin, FITC.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TRPM5 antibody was raised against synthetic peptide located between aa247-296 of human TRPM5 (Q9NZQ8, NP_055370). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey, Galago, Marmoset, Elephant, Dog, Horse (92%); Mouse (85%); Rat (78%).
Epitope
aa247-296
Specificity
Human TRPM5
Usage
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
100 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

TRPM5 antibody LS-C80116 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TRPM5 antibody LS-C80116 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

TRPM5 antibody LS-C80116 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TRPM5 antibody LS-C80116 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

TRPM5 antibody LS-C80116 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TRPM5 antibody LS-C80116 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

TRPM5 antibody LS-C80116 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TRPM5 antibody LS-C80116 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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