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Catalog Number Size Price
LS-C312898-10 10 µg $318 
LS-C312898-100 100 µg $470 
TRPC3 Antibody - TRPC3 antibody. IHC(P): Human Rectal Cancer Tissue.
TRPC3 Antibody - TRPC3 antibody. IHC(P): Rat Brain Tissue.
TRPC3 Antibody - Western blot analysis of TRPC3 using anti-TRPC3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 22RV1 whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRPC3 at approximately 110KD. The expected band size for TRPC3 is at 93KD.
TRPC3 Antibody - Anti-TRPC3 antibody, Western blotting All lanes: Anti TRPC3 at 0.5ug/ml Lane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Lung Tissue Lysate at 50ugLane 3: Rat Intestine Tissue Lysate at 50ugLane 4: Rat Ovary Tissue Lysate at 50ugLane 5: U87 Whole Cell Lysate at 40ugLane 6: A549 Whole Cell Lysate at 40ugLane 7: COLO320 Whole Cell Lysate at 40ugLane 8: SW620 Whole Cell Lysate at 40ugLane 9: HELA Whole Cell Lysate at 40ugPredicted bind size: 97KD Observed bind size: 120KD
TRPC3 Antibody - WB of TRPC3 antibody. Lane 1: Rat Liver Tissue Lysate. Lane 2: Rat Lung Tissue Lysate. Lane 3: Rat Intestine Tissue Lysate. Lane 4: Rat Ovary Tissue Lysate. Lane 5: U87 Cell Lysate. Lane 6: A549 Cell Lysate. Lane 7: COLO320 Cell Lysate. Lane 8: SW620 Cell Lysate. Lane 9: HELA Cell Lysate.
TRPC3 Antibody - TRPC3 antibody. IHC(P): Human Rectal Cancer Tissue.
TRPC3 Antibody - TRPC3 antibody. IHC(P): Rat Brain Tissue.
TRPC3 Antibody - Western blot analysis of TRPC3 using anti-TRPC3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 22RV1 whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRPC3 at approximately 110KD. The expected band size for TRPC3 is at 93KD.
TRPC3 Antibody - Anti-TRPC3 antibody, Western blotting All lanes: Anti TRPC3 at 0.5ug/ml Lane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Lung Tissue Lysate at 50ugLane 3: Rat Intestine Tissue Lysate at 50ugLane 4: Rat Ovary Tissue Lysate at 50ugLane 5: U87 Whole Cell Lysate at 40ugLane 6: A549 Whole Cell Lysate at 40ugLane 7: COLO320 Whole Cell Lysate at 40ugLane 8: SW620 Whole Cell Lysate at 40ugLane 9: HELA Whole Cell Lysate at 40ugPredicted bind size: 97KD Observed bind size: 120KD
TRPC3 Antibody - WB of TRPC3 antibody. Lane 1: Rat Liver Tissue Lysate. Lane 2: Rat Lung Tissue Lysate. Lane 3: Rat Intestine Tissue Lysate. Lane 4: Rat Ovary Tissue Lysate. Lane 5: U87 Cell Lysate. Lane 6: A549 Cell Lysate. Lane 7: COLO320 Cell Lysate. Lane 8: SW620 Cell Lysate. Lane 9: HELA Cell Lysate.
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Polyclonal Rabbit anti‑Human TRPC3 Antibody (aa836‑851, IHC, WB) LS‑C312898

Polyclonal Rabbit anti‑Human TRPC3 Antibody (aa836‑851, IHC, WB) LS‑C312898

Note: This antibody replaces LS-C389384
Antibody:
TRPC3 Rabbit anti-Human Polyclonal (aa836-851) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Rabbit, Sheep, Chicken, Zebrafish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312898-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TRPC3 Rabbit anti-Human Polyclonal (aa836-851) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Rabbit, Sheep, Chicken, Zebrafish
Format:
Unconjugated, Unmodified

Specifications

Description
TRPC3 antibody LS-C312898 is an unconjugated rabbit polyclonal antibody to TRPC3 (aa836-851) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Human TRPC3
Synonyms
TRPC3 | HTrp-3 | HTrp3 | TRP3 | Transient receptor protein 3 | TRP-3 | Trrp3
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Rabbit, Sheep, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human TRPC3(836-851 aa HSFNSILNQPTRYQQI), identical to the related rat and mouse sequences.
Epitope
aa836-851
Specificity
Expressed predominantly in brain and at much lower levels in ovary, colon, small intestine, lung, prostate, placenta and testis.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TRPC3
Q13507 NM_003305 NP_003296.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024