Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Specificity
TRITC. The reactivity of the antiserum is directed to TRITC as tested in direct binding enzyme immunoassay, ELISA and immunoblotting. Cross-reactivity This antiserum has not been tested for cross-reactivity with other fluorescent compounds.
Usage
This product is intended for use to enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to TRITC. The phenomenon of a weak reaction of a monoclonal antibody is e. g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the TRITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:510 to 1:40; in ELISA from 1:100 upwards; in Western blotting from 1:200 upwards. These data should be interpreted as general recommendations only.
Reconstitution
Reconstitute with 0.5ml distilled H2O.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee