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Anti-TRH Antibody (C-Terminus) LS-C82035

Catalog	Size	Price
LS-C82035-50	50 µg	Unavailable

Related Products

10 TRH Antibodies

Most Popular TRH Antibodies

Antibody:	Rabbit Polyclonal (IgG) to Human TRH
Reactivity:	Human, Monkey, Bat, Bovine, Dog, Guinea pig, Horse, Pig
Application:	Western blot
Format:	Unconjugated

Transfected 293T cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Anti-TRH Antibody LS-C296647

Antibody:	Rabbit Polyclonal (IgG) to Human TRH
Reactivity:	Human
Application:	Western blot
Format:	Unconjugated

Anti-TRH Antibody LS-C296648

Antibody:	Rabbit Polyclonal (IgG) to Mouse TRH
Reactivity:	Mouse
Application:	Western blot
Format:	Unconjugated

Anti-TRH Antibody (Biotin) LS-C298878

Antibody:	Rabbit Polyclonal (IgG) to Mouse TRH
Reactivity:	Mouse
Application:	Western blot
Format:	Biotin Conjugated

Anti-TRH Antibody (aa157-191) LS-C392918

Antibody:	Rabbit Polyclonal to Human TRH
Reactivity:	Human
Application:	Western blot, ELISA
Format:	Unconjugated

All lanes: Anti-TRH Antibody (C-Term) at 1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: human liver lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 27 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Antibody:	Rabbit Polyclonal (IgG) to Human TRH
Reactivity:	Human, Monkey, Bat, Bovine, Dog, Guinea pig, Horse, Pig
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

Details

Target:	Human TRH
Host:	Rabbit
Reactivity:	Human, Monkey, Bat, Bovine, Dog, Guinea pig, Horse, Pig (tested or 100% immunogen sequence identity)
Predicted:	Zebrafish (at least 90% immunogen sequence identity)
Clonality:	IgG Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

TRH antibody was raised against synthetic peptide from C-Terminus of human TRH (P20396, NP_009048). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Medaka (100%); Zebrafish (92%); Turkey (91%); Smelt, Stickleback, Pufferfish (90%); Guinea pig (84%); Zebra finch, Salmon (83%).

Blocking Peptide:

LS-E10724 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C82035. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

C-Terminus

Specificity:

Human TRH

Usage:

ELISA titer using peptide based assay: 1:2500. Western Blot: Suggested dilution at 0.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Presentation:	Lyophilized from PBS, 2% sucrose.
Reconstitution:	50 µl Distilled Water.
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About TRH

Uniprot: P20396 NCBI: NM_007117 NP_009048.1

Synonyms: TRH Antibody, Thyroliberin Antibody, Thyrotropin-releasing hormone Antibody, Prothyroliberin Antibody

Description: Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems. May promote hair shaft elongation, prolonge the hair cycle growth phase (anagen) and antagonized its termination by TGFB2. May also increase proliferation and inhibited apoptosis of hair matrix keratinocytes.

(More About TRH)

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Western blot

Transfected 293T cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 4/24/2018

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