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TRH Antibody (C‑Terminus) LS‑C82035
TRH antibody LS-C82035 is an unconjugated rabbit polyclonal antibody to TRH from human, bat, bovine and other species. Validated for WB.
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Species:
Mouse, Human
Applications:
IHC, ICC, Western blot, Immunoprecipitation
Species:
Mouse, Human
Applications:
IHC, ICC, Western blot, Immunoprecipitation
Species:
Mouse, Human
Applications:
IHC, ICC, Western blot, Immunoprecipitation
Species:
Mouse, Human
Applications:
IHC, ICC, Western blot, Immunoprecipitation
Species:
Mouse, Human
Applications:
IHC, ICC, Western blot, Immunoprecipitation
Product Description
TRH antibody LS-C82035 is an unconjugated rabbit polyclonal antibody to TRH from human, bat, bovine and other species. Validated for WB.
About TRH
Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems. May promote hair shaft elongation, prolonge the hair cycle growth phase (anagen) and antagonized its termination by TGFB2. May also increase proliferation and inhibited apoptosis of hair matrix keratinocytes.
P20396
NM_007117
NP_009048.1
TRH Antibody, Thyroliberin Antibody, Thyrotropin-releasing hormone Antibody, Prothyroliberin Antibody
Specifications
Target
Human TRH
Host
Rabbit
Reactivity
Human, Monkey, Bat, Bovine, Dog, Guinea pig, Horse, Pig
(tested or 100% immunogen sequence identity)
Predicted
Zebrafish (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- Western blot
- (applications tested for the base form of this product only)
Immunogen
TRH antibody was raised against synthetic peptide from C-Terminus of human TRH (P20396, NP_009048). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Medaka (100%); Zebrafish (92%); Turkey (91%); Smelt, Stickleback, Pufferfish (90%); Guinea pig (84%); Zebra finch, Salmon (83%).
Blocking Peptide
LS-E10724 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C82035. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-C82035. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human TRH
Usage
ELISA titer using peptide based assay: 1:2500. Western Blot: Suggested dilution at 0.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS, 2% sucrose.
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Western blot
Transfected 293T cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
Transfected 293T cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
Transfected 293T cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
Transfected 293T cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From:
Date Requested: 6/24/2018
Date Requested: 6/24/2018
