Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Protein A purified
IHC - Paraffin, Western blot
Specificity and Use
TRADD antibody was raised against a synthetic peptide from C-terminus of human TRADD.
Recognizes TRADD (TNF R1 Associated Death Domain). Species cross-reactivity: human.
Western Blot (Ab 5-10 ug/ml for 2hrs at RT). Immunohistochemistry (formalin/paraffin) (Use Ab at 5-10 ug/ml for 30 min at RT). Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Hey cells. Angiosarcoma.
10 mM PBS, pH 7.4.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12: acts by interacting with TRIP12, leading to disrupt interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A (By similarity). Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD.