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Anti-TOCA1 / FNBP1L Antibody (C-Terminus) IHC-plus™ LS-B3214

Note: This antibody replaces LS-C53219

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular TOCA1 / FNBP1L Antibodies

Anti-TOCA1 / FNBP1L Antibody (N-Terminus) LS-C53220
Rabbit Polyclonal to Human TOCA1 / FNBP1L
Human, Mouse, Rat
Western blot
Unconjugated
Immunofluorescence Image
Anti-TOCA1 / FNBP1L Antibody (C-Terminus) IHC-plus™ LS-B3214
Rabbit Polyclonal to Human TOCA1 / FNBP1L
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human TOCA1 / FNBP1L
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human TOCA1 / FNBP1L
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • ELISA

Specificity and Use

TOCA1 / FNBP1L antibody was raised against 17 amino acid peptide near the carboxy terminus of the human TOCA-1.
LS-E30408 - Liquid - 50 µg - $145.00
C-Terminus
Immunohistochemistry: LS-B3214 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3214 was determined to be 2.5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About TOCA1 / FNBP1L

Q5T0N5 NM_017737 NP_060207.2

FNBP1L Antibody, C1orf39 Antibody, Formin-binding protein 1-like Antibody, Formin binding protein 1-like Antibody, TOCA1 Antibody, Toca-1 Antibody

Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. May bind to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promote membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by activating the WASL/N-WASP-WASPIP/WIP complex, the predominant form of WASL/N-WASP in cells. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles.

Immunohistochemistry

Immunohistochemistry
Anti-FNBP1L / Toca-1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3214 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-FNBP1L / Toca-1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3214 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of TOCA-1 in Human Brain cells with TOCA-1 antibody at 20 ug/ml.

Western blot

Western blot
Western blot of TOCA-1 in mouse brain tissue lysate with in (A) the absence and (B) the presence of blocking peptide with TOCA-1 antibody at 1 ug/ml. Below: Immunohistochemistry of TOCA-1 in human brain tissue with TOCA-1 antibody at 2.5 ug/ml.

Requested From: United States
Date Requested: 12/3/2016

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