Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone V1q] (IgD) to Mouse TNF Alpha
Flow Cytometry, Functional Assay
Rat Monoclonal [clone MP6-XT3] (IgG1) to Mouse TNF Alpha
Western blot, Flow Cytometry, ELISA, Functional Assay
Unconjugated, Low Endotoxin
Mouse TNF Alpha
Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [MP6-XT3]
Protein G purified
ELISA (1 - 5 µg/ml)
Specificity and Use
TNF Alpha antibody was raised against recombinant murine TNF-alpha expressed in E. coli.
Recognizes mouse tumor necrosis factor alpha (TNF alpha), also known as TNFSF2. This 17kD cytokine is produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26kD integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immunoregulatory role. Is reported to neutralize the effects of mouse TNF alpha.
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
TNF Alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.