Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone V1q] (IgD) to Mouse TNF Alpha
Flow Cytometry, Functional Assay
Rat Monoclonal (IgG1) to Mouse TNF Alpha
Western blot, Neutralization
Mouse TNF Alpha
Mouse (tested or 100% immunogen sequence identity)
Ion exchange chromatography
Western blot (1 µg/ml)
Neutralization (3 µg/ml)
Specificity and Use
TNF Alpha antibody was raised against recombinant mouse TNFa expressed in E. coli.
Binds and neutralizes murine TNFa. Does not recognize human or rat TNFa.
Suitable for use in Dot Blot and Neutralization. Dot Blot: 1 ug/ml detected 5 ng recombinant murine TNFa. Neutralization: 3 ug/ml neutralized 50% of the cytotoxic effect of 50pg/ml recombinant mouse TNFa on actinomycin D treated L929 cells. The antibody concentration needed to neutralize the bioactivity of TNFa will depend on cell type, incubation conditions and biological response.
0.01 M Tris-glycine, pH 7.4, 0.15 M sodium chloride.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
TNF Alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.