Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone V1q] (IgD) to Mouse TNF Alpha
Flow Cytometry, Functional Assay
Rabbit Polyclonal (IgG) to Human TNF Alpha
Western blot, Flow Cytometry, ELISA, Neutralization
Human TNF Alpha
Human, Monkey (tested or 100% immunogen sequence identity)
Western blot (1:10)
Flow Cytometry (1:10)
Specificity and Use
Reacts with human natural and recombinant TNF-alpha as assessed by ELISA. Inhibits the biological activity of human natural and recombinant TNF-alpha as determined with L929 and WEHI cells in a cytotoxicity assay. Cross-reacts with rhesus and cynomolgus natural TNF-alpha and lacks cross-reactivity with human lymphotoxin.
Suitable for use in ELISA, Western Blot, Flow Cytometry and Neutralization studies. Flow Cytometry: 1:10. Western Blot: 1:10. Neutralization assays: sterile filter before use.
TNF Alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.