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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TNF Alpha Antibody (Biotin) LS‑C104788
Note: This antibody replaces LS-C54301, LS-C42530, LS-C18315
Antibody
TNF Alpha antibody LS-C104788 is a biotin-conjugated goat polyclonal antibody to human TNF Alpha. Validated for ELISA, SwELISA and WB.
Catalog
Size
Price
LS-C104788-25
25 µg
Unavailable
LS-C104788-50
50 µg
Unavailable

Popular TNF Alpha Products

Antibody
Species: Mouse
Applications: Western blot
Anti-TNF Alpha antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2123 concentration 5 ug/ml.
Species: Human, Monkey, Mouse, Guinea pig
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Antibody
Species: Human, Monkey, Cynomolgus
Applications: Western blot, ELISA
Anti-TNF Alpha antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6067 concentration 5 ug/ml.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Mouse, Hamster
Applications: Flow Cytometry, Functional Assay

Product Description

TNF Alpha antibody LS-C104788 is a biotin-conjugated goat polyclonal antibody to human TNF Alpha. Validated for ELISA, SwELISA and WB.
About TNF Alpha
TNF Alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. P01375 NM_000594 NP_000585.2

TNF Antibody, APC1 protein Antibody, Cachectin Antibody, DIF Antibody, TNF Alpha Antibody, Tumor necrosis factor-alpha Antibody, TNF-alpha Antibody, TNF-a Antibody, TNFA Antibody, Tumor necrosis factor Antibody, TNF, macrophage-derived Antibody, TNF, monocyte-derived Antibody, TNFSF2 Antibody


Specifications

Target
Human TNF Alpha
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (1 µg/ml)
  • Sandwich ELISA
Immunogen
TNF Alpha antibody was raised against e.coli derived recombinant Human TNF-alpha.
Specificity
Human TNF-alpha
Usage
ELISA: To detect Human TNF-alpha by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-alpha. To detect Human TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TNF-alpha (LS-C104718) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-alpha. Western Blot: To detect Human TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Sterile PBS containing 0.1% BSA
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/20/2018

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