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Anti-TNF Alpha Antibody (Biotin) LS-C104788

Note: This antibody replaces LS-C54301, LS-C42530, LS-C18315
Catalog Size Price
LS-C104788-25 25 µg Unavailable
LS-C104788-50 50 µg Unavailable

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421 TNF Alpha Antibodies

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Anti-TNF Alpha Antibody LS-C18838
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Anti-TNF Alpha Antibody (clone 52B83) IHC-plus™ LS-B2123
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Anti-TNF Alpha Antibody LS-C132747
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Anti-TNF Alpha Antibody (clone M 1 C4) IHC-plus™ LS-B6067
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Anti-TNF Alpha Antibody (clone V1q) LS-C139854
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100% Guaranteed
Goat Polyclonal to Human TNF Alpha
Human
Western blot, ELISA, Sandwich ELISA
Biotin Conjugated

Details

Human TNF Alpha
Goat
Human (tested or 100% immunogen sequence identity)
Polyclonal
Biotin
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (1 µg/ml)
  • Sandwich ELISA

Specificity and Use

TNF Alpha antibody was raised against e.coli derived recombinant Human TNF-alpha.
Human TNF-alpha
ELISA: To detect Human TNF-alpha by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-alpha. To detect Human TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TNF-alpha (LS-C104718) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-alpha. Western Blot: To detect Human TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

Lyophilized from PBS, pH 7.2
Sterile PBS containing 0.1% BSA
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About TNF Alpha

P01375 NM_000594 NP_000585.2

TNF Antibody, APC1 protein Antibody, Cachectin Antibody, DIF Antibody, TNF Alpha Antibody, Tumor necrosis factor-alpha Antibody, TNF-alpha Antibody, TNF-a Antibody, TNFA Antibody, Tumor necrosis factor Antibody, TNF, macrophage-derived Antibody, TNF, monocyte-derived Antibody, TNFSF2 Antibody

TNF Alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.

Requested From: 
Date Requested: 8/22/2017

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