Human TNC / Tenascin C

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

T2H5

Unconjugated

Purified

Unmodified

T2H5 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a BALB/c mouse immunized with full length native tenascin protein purified from a homogenate of a human mammary tumor specimen.

A 265 kD protein. Tenascin C contains an extensive number of potential glycosylation sites, which may explain its migration at a higher molecular weight than predicted. Human tonsilar tissue can be used as a positive control in immunocytochemistry and immunohistochemistry.

• IHC
• IHC - Paraffin
• IHC - Frozen
• ICC
• Western blot
• Immunoprecipitation

Tenascin is a high molecular weight, multifunctional, extracellular matrix glycoprotein expressed in association with mesenchymal-epithelial interactions during development. It has been described under a variety of names, i. e. cytotactin, hexabrachion protein, J1, myotendinous antigen and glioma mesenchymal extracellular matrix. In undifferentiated tumors it is also detectable in the neovasculature and stroma. The tenascin molecule is a disulfide-linked hexamer. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted. For example, this extracellular matrix protein is implicated in guidance of migrating neurons as well as axons during neural development as well as neuronal regeneration. In in vitro studies it promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. The cellular ligands for tenascin include integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.

PBS, 0.09% Sodium Azide

Store at 4°C, or in small aliquots at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P24821 NCBI: NM_002160 NP_002151.2