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TMEM254 / C10orf57 Antibody (aa36‑85) LS‑C102349
C10orf57 antibody LS-C102349 is an unconjugated rabbit polyclonal antibody to C10orf57 (TMEM254) from human, horse and pig. Validated for WB.
Catalog
Size
Price
LS-C102349-50
50 µg
Unavailable

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Product Description

C10orf57 antibody LS-C102349 is an unconjugated rabbit polyclonal antibody to C10orf57 (TMEM254) from human, horse and pig. Validated for WB.

Specifications

Target
Human TMEM254 / C10orf57
Host
Rabbit
Reactivity
Human, Horse, Pig (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat, Bovine (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa36-85 of human TMEM254 (Q8TBM7, NP_079401). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Horse (100%); Monkey, Bovine, Bat (92%); Galago, Marmoset, Mouse, Rat, Panda, Dog, Pig, Guinea pig, Platypus (85%).
Blocking Peptide
LS-E18030 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C102349. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa36-85
Specificity
Human C10orf57
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

C10orf57 antibody LS-C102349 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
C10orf57 antibody LS-C102349 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

C10orf57 antibody LS-C102349 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
C10orf57 antibody LS-C102349 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

C10orf57 antibody LS-C102349 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
C10orf57 antibody LS-C102349 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

C10orf57 antibody LS-C102349 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
C10orf57 antibody LS-C102349 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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