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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C148165-100 100 µg (0.5 mg/ml) $365 
TLR4 Antibody - Cell surface flow cytometry of TLR4 on ThP1 cells using TLR4 antibody at 2 ug/10^6 cells. The shaded histogram represents ThP1 cells only, green represents isotype control antibody, and red represents TLR4 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
TLR4 Antibody - Intracellular flow cytometry of TLR4 in human PBMCs using TLR4 antibody at 0.5 ug/10^6 cells. The shaded histogram represents cells without TLR4 antibody; green represents the isotype control; red represents TLR4 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
TLR4 Antibody - Cell surface flow cytometry of TLR4 on ThP1 cells using TLR4 antibody at 2 ug/10^6 cells. The shaded histogram represents ThP1 cells only, green represents isotype control antibody, and red represents TLR4 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
TLR4 Antibody - Intracellular flow cytometry of TLR4 in human PBMCs using TLR4 antibody at 0.5 ug/10^6 cells. The shaded histogram represents cells without TLR4 antibody; green represents the isotype control; red represents TLR4 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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TLR4 Antibody (clone HTA125, PE) LS‑C148165

TLR4 Antibody (clone HTA125, PE) LS‑C148165

Antibody:
Mouse Monoclonal to Human TLR4
Application:
Flo
Reactivity:
Human, Dog
Format:
Phycoerythrin, Unmodified
Price
Catalog Number
$365 
LS-C148165-100
100 µg
0.5 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human TLR4
Application:
Flo
Reactivity:
Human, Dog
Format:
Phycoerythrin, Unmodified

Specifications

Description
TLR4 antibody LS-C148165 is a PE-conjugated mouse monoclonal antibody to TLR4 from human and dog. Validated for Flow.
Target
Human TLR4
Host
Mouse
Reactivity
Human, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
HTA125
Conjugations
Phycoerythrin
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Flow Cytometry (1 - 2 µg/10E6 cells)
Immunogen
This antibody was developed by immunizing mice with Ba/F3 cell line expressing human TLR4 cell surface antigen (9).
Specificity
This recognizes the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. This has been demonstrated to block activation of monocytes with LPS.
Usage
Flow: Cell Surface, Intracellular. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.05% Sodium Azide, 0.05% BSA
Storage
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for up to 6 months at 4°C. Protect from light.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TLR4
O00206 NM_138554 O00206

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Requested From: United States
Date Requested: 12/7/2019