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Anti-TLR4 Antibody (aa24-631) LS-C150087


Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular TLR4 Antibodies

Anti-TLR4 Antibody (clone HTA125, PE, Cy7) LS-C107190
Mouse Monoclonal [clone HTA125] (IgG2a,k) to Human TLR4
Flow Cytometry
PE, Cy7 Conjugated
Flow Cytometry Image
Anti-TLR4 Antibody (Internal) IHC-plus™ LS-A9704
Rabbit Polyclonal to Human TLR4
IHC - Paraffin
Immunohistochemistry Image
Anti-TLR4 Antibody (aa100-200, clone 76B357.1) IHC-plus™ LS-B2070
Mouse Monoclonal [clone 76B357.1] (IgG2b,k) to Human TLR4
Human, Mouse, Rat
IHC - Paraffin, Western blot, Flow Cytometry
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human TLR4
IHC - Paraffin, ICC, Western blot, Flow Cytometry, Neutralization


Human TLR4
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • IHC - Paraffin (5 - 15 µg/ml)
  • ICC (5 - 15 µg/ml)
  • Western blot (1 µg/ml)
  • Flow Cytometry
  • Neutralization

Specificity and Use

TLR4 antibody was raised against mouse myeloma cell line NS0-derived recombinant human TLR4 Glu24-Lys631 Accession # O00206.
Detects human TLR4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with.
Western Blot, 1 µg/ml. Flow Cytometry, 2.5 µg/106 cells, Human peripheral blood monocytes. Immunocytochemistry, 5-15 µg/ml. Immunohistochemistry, 5-15 µg/ml. Neutralization, Measured by its ability to neutralize LPS-induced IL-8 secretion in the HEK293 human embryonic kidney cell line. co-transfected with human TLR4 and MD-2. The Neutralization Dose (ND50) is typically 1.5-7.5 µg/ml in the presence of 75 ng/ml Lipopolysacharide (LPS).


Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About TLR4

O00206 NM_138554 O00206

TLR4 Antibody, CD284 Antibody, CD284 antigen Antibody, Homolog of Drosophila toll Antibody, TLR-4 Antibody, TOLL Antibody, ARMD10 Antibody, Toll-like receptor 4 Antibody, HToll Antibody

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni2+. Responses triggered by Ni2+ require non-conserved histidines and are, therefore, species-specific.


TLR4 in Human Prostate. TLR4 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human TLR4 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml overnight at 4°C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).


TLR4 in Human PBMCs. TLR4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human TLR4 Antigen Affinity-purified Polyclonal Antibody 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (yellow) and counterstained with DAPI (blue).

Western blot

Western blot
Detection of Human TLR4 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human TLR4 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. Specific bands were detected for TLR4 at approximately 110 kD and 130 kD (as indicated). This experiment was conducted under reducing conditions.


IL-8 Secretion Induced by LPS and Neutralization by Human TLR4 Antibody. Lipopolysacharide (LPS) stimulates IL-8 secretion in the HEK293 human embryonic kidney cell line co-transfected with human TLR4 and MD-2, in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL-8 Quantikine ELISA Kit. IL-8 secretion elicited by LPS (75 ng/ml) is neutralized (green line) by increasing concentrations of Goat Anti-Human TLR4 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 1.5-7.5 ug/ml.

Requested From: United States
Date Requested: 10/24/2016

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