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Anti-TGM3 / Transglutaminase 3 Antibody (aa2-693) LS-C150089

Ordering

Wt. Vol. Conc. Price
100 µg - - $375
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TGM3 / Transglutaminase 3 Antibodies

Anti-TGM3 / Transglutaminase 3 Antibody (clone H3) LS-C144539
Mouse Monoclonal [clone H3] (IgG1,k) to Human TGM3 / Transglutaminase 3
Human
IHC, Western blot
Unconjugated
Anti-TGM3 / Transglutaminase 3 Antibody (aa598-610) LS-C154878
Goat Polyclonal to Human TGM3 / Transglutaminase 3
Human
Western blot, ELISA
Unconjugated
Western blot Image
Anti-TGM3 / Transglutaminase 3 Antibody (aa468-693, Biotin) LS-C300413
Rabbit Polyclonal (IgG) to Human TGM3 / Transglutaminase 3
Human
Western blot, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Human TGM3 / Transglutaminase 3
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human TGM3 / Transglutaminase 3
Sheep
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

TGM3 / Transglutaminase 3 antibody was raised against s. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 3/TGM3 Ala2-Glu693 Accession # NP_003236.
aa2-693
Detects human Transglutaminase 3/TGM3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About TGM3 / Transglutaminase 3

Q08188 NM_003245 NP_003236.3

TGM3 Antibody, TGE Antibody, Transglutaminase 3 Antibody, Transglutaminase E Antibody, Transglutaminase-3 Antibody, TGase-3 Antibody, TG(E) Antibody, TGase E Antibody

Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes.

Immunohistochemistry

Immunohistochemistry
Transglutaminase 3/TGM3 in Human Skin. Transglutaminase 3/TGM3 was detected in immersion fixed paraffin-embedded sections of human skin using Human Transglutaminase 3/TGM3 Antigen Affinity-purified Polyclonal Antibody at 3 ug/ml overnight at 4°C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cells in the hair follicle.

Western blot

Western blot
Detection of Human and Mouse Transglutaminase 3/TGM3 by Western Blot. Western blot shows lysates of B16-F1 mouse melanoma cell line, Bowes human melanoma cell line, and human skin tissue. PVDF Membrane was probed with 1 ug/ml of Human Transglutaminase 3/TGM3 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. For additional reference, Recombinant Human Transglutaminase 3/TGM3 (10 ng/lane) uncleaved and cleaved by recombinant human Cathepsin were included. Specific bands were detected for uncleaved Transglutaminase 3/TGM3 at approximately 77 kD (as indicated) and Transglutaminase 3/TGM3 cleavage products at approximately 50 and 30 kD (as indicated). This experiment was conducted under reducing conditions.

Requested From: United States
Date Requested: 12/9/2016

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