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Anti-TFAP4 / AP-4 Antibody (clone 7A10) LS-C133479

Catalog Size Price
LS-C133479-100 100 µg Unavailable

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Immunoperoxidase of monoclonal antibody to TFAP4 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1 ug/ml]
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100% Guaranteed
Mouse Monoclonal [clone 7A10] (IgG2a,k) to Human TFAP4 / AP-4
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IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human TFAP4 / AP-4
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [7A10]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

TFAP4 / AP-4 antibody was raised against tFAP4 (NP_003214, 93 a.a. ~ 193 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human TFAP4
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.4
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
For research use only.

About TFAP4 / AP-4

Q01664 NM_003223 NP_003214.1

TFAP4 Antibody, AP-4 Antibody, BHLHc41 Antibody, Transcription factor AP-4 Antibody

Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 [PubMed 2833704]; Hu et al., 1990 [PubMed 2123466]).

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to TFAP4 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1 ug/ml]
Immunoperoxidase of monoclonal antibody to TFAP4 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1 ug/ml]

Western blot

TFAP4 monoclonal antibody (M03), clone 7A10 Western blot of TFAP4 expression in HeLa NE.
TFAP4 monoclonal antibody (M03), clone 7A10 Western blot of TFAP4 expression in HeLa NE.

Western blot

Western blot of TFAP4 expression in transfected 293T cell line by TFAP4 monoclonal antibody (M03), clone 7A10.
Western blot of TFAP4 expression in transfected 293T cell line by TFAP4 monoclonal antibody (M03), clone 7A10.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged TFAP4 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged TFAP4 is approximately 0.1 ng/ml as a capture antibody.

RNA interference

Western blot of TFAP4 over-expressed 293 cell line, cotransfected with TFAP4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TFAP4 monoclonal antibody, clone 7A10. GAPDH ( 36.1 kD ) used as specificity and loading control.
Western blot of TFAP4 over-expressed 293 cell line, cotransfected with TFAP4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TFAP4 monoclonal antibody, clone 7A10. GAPDH ( 36.1 kD ) used as specificity and loading control.

Requested From: 
Date Requested: 5/25/2018

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