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Anti-TDP-43 / TARDBP Antibody (Internal) IHC-plus™ LS-B2223

Note: This antibody replaces LS-C34725, LS-C132363

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TDP-43 / TARDBP Antibodies

Anti-TDP-43 / TARDBP Antibody (clone 2E2-D3) IHC-plus™ LS-B4521
Mouse Monoclonal [clone 2E2-D3] (IgG1,k) to Human TDP-43 / TARDBP
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA, RNA interference
Unconjugated
Immunohistochemistry Image
Anti-TDP-43 / TARDBP Antibody LS-C124633
Rat Monoclonal (IgG2a,k) to Human TDP-43 / TARDBP
Human
IHC, Western blot, ELISA
Unconjugated
Anti-TDP-43 / TARDBP Antibody (C-Terminus) LS-C144920
Rabbit Polyclonal to Mouse TDP-43 / TARDBP
Mouse
IHC, Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human TDP-43 / TARDBP
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated

Details

Human TDP-43 / TARDBP
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

TDP-43 / TARDBP antibody was raised against synthetic peptide from human TARDBP.
LS-E30372 - Liquid - 50 µg - $145.00
Internal
An 18 amino acid peptide from near the center of human TDP43. This antibody recognizes only the longer of the two isoforms.
Immunohistochemistry: LS-B2223 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2223 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About TDP-43 / TARDBP

Q13148 NM_007375 NP_031401.1

TARDBP Antibody, ALS10 Antibody, TAR DNA-binding protein-43 Antibody, TDP-43 Antibody, TAR DNA binding protein Antibody, TAR DNA-binding protein 43 Antibody, TDP43 Antibody

DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division.

Immunohistochemistry

Immunohistochemistry
Anti-TARDBP antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2223 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry
Immunocytochemistry of TDP43 in HeLa cells with TDP43 antibody at 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of TDP43 in HeLa cells with TDP43 antibody at 20 ug/ml.

Western blot

Western blot
Western blot of TDP43 in L1210 cell lysate with TDP43 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.

Requested From: United States
Date Requested: 12/4/2016

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