Target
Mouse TCP1
Synonyms
TCP1 | CCTA | CCT-alpha | D6S230E | T-complex 1 | TCP-1-alpha | Tailless complex polypeptide 1 | CCT1
Reactivity
Mouse, Human, Monkey, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Drosophila, C. elegans, Plant
(tested or 100% immunogen sequence identity)
Immunogen
A purified recombinant mouse TCP-1 construct encoding the C-Terminus half of the 1.8 kb full-length Tcp1b gene expressed in E. coli.
Specificity
Recognizes an epitope in the C- terminus of mouse TCP-1a (465-AKLRA) and will detect an ~60 kD protein, corresponding to the apparent molecular mass of TCP1 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, guinea pig, hamster, monkey, pig, and rabbit origins, as well as Saccharomyces cerevisiae (weakly, consistent with the epitope sequence being AKLRS), Drosophila, Schizosaccharomyces pombe and C. elegans. In C. elegans CTA-191 reacts with TCP-1a and another CCT subunit (2). In plants, CTA-191 recognizes TCP -1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP-1a over the region of the epitope AKLRA. It has also been shown that CTA-191 reacts with a subunit of a specialized chaperonin which folds phytochrome (3). Cross reactivity with human Hsp60 has been observed with this antibody in immunoblot analysis.