TCG4 antibody LS-C18042 is an unconjugated mouse monoclonal antibody to TCG4 (TCF7L2) from human and mouse. Validated for ELISA, ICC, IHC, IP and WB.
Human TCF7L2 / TCG4
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot (0.1 - 2 µg/ml)
- (applications tested for the base form of this product only)
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TCF7L2 / TCG4 antibody was raised against his-tagged fusion protein corresponding to residues 31-331 of human TCF-4.
Recognizes human TCF-4 at 66kD in Cos-1 cells expressing transfected TCF-4. Species cross-reactivity: mouse.
Suitable for use in Western Blot, ELISA, Supershift Assay, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot: 0.1-2 ug/ml. Detected TCF-4 in RIPA lysates of Cos-1 cells expressing transfected TCF-4. Supershift: Reported to supershift TCF-4: DNA complexes in electrophoretic mobility shift assays (EMSA). Immunoprecipitation: Reported to immunoprecipitate TCF-4 from SW620 whole cell lysates as a complex with b-Catenin. Immunocytochemistry/Immunohistochemistry: Reported to show positive immunostaining for TCF-4 in cells fixed with 4% paraformaldehyde/PBS. Positive control: DLD1 (human colorectal adenocarcinoma), Cos-1 cells or HCT116 (human colorectal carcinoma) cells.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.