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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TCF7L2 / TCG4 Antibody (aa31‑331) LS‑C18042 Best Seller
TCG4 antibody LS-C18042 is an unconjugated mouse monoclonal antibody to TCG4 (TCF7L2) from human and mouse. Validated for ELISA, ICC, IHC, IP and WB.
Antibody
Catalog
Size
Price
LS-C18042-200
200 µg (1 mg/ml)
$680

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Product Description

TCG4 antibody LS-C18042 is an unconjugated mouse monoclonal antibody to TCG4 (TCF7L2) from human and mouse. Validated for ELISA, ICC, IHC, IP and WB.
About TCF7L2 / TCG4
Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Q9NQB0 NM_030756 NP_110383.2

TCF7L2 Antibody, HMG box transcription factor 4 Antibody, HTCF-4 Antibody, T-cell factor-4 variant X2 Antibody, T-cell factor 4 Antibody, Transcription factor 7-like 2 Antibody, TCF-4 Antibody


Specifications

Target
Human TCF7L2 / TCG4
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • ICC
  • Western blot (0.1 - 2 µg/ml)
  • Immunoprecipitation
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TCF7L2 / TCG4 antibody was raised against his-tagged fusion protein corresponding to residues 31-331 of human TCF-4.
Epitope
aa31-331
Specificity
Recognizes human TCF-4 at 66kD in Cos-1 cells expressing transfected TCF-4. Species cross-reactivity: mouse.
Usage
Suitable for use in Western Blot, ELISA, Supershift Assay, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot: 0.1-2 ug/ml. Detected TCF-4 in RIPA lysates of Cos-1 cells expressing transfected TCF-4. Supershift: Reported to supershift TCF-4: DNA complexes in electrophoretic mobility shift assays (EMSA). Immunoprecipitation: Reported to immunoprecipitate TCF-4 from SW620 whole cell lysates as a complex with b-Catenin. Immunocytochemistry/Immunohistochemistry: Reported to show positive immunostaining for TCF-4 in cells fixed with 4% paraformaldehyde/PBS. Positive control: DLD1 (human colorectal adenocarcinoma), Cos-1 cells or HCT116 (human colorectal carcinoma) cells.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 8/17/2018

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