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Anti-TCF7L1 / TCF-3 Antibody (aa35-84) IHC-plus™ LS-B3476

Note: This antibody replaces LS-C101336

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TCF7L1 / TCF-3 Antibodies

Anti-TCF7L1 / TCF-3 Antibody (aa35-84) IHC-plus™ LS-B3476
Rabbit Polyclonal (IgG) to Human TCF7L1 / TCF-3
Human, Mouse, Rat, Bovine, Guinea pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-TCF7L1 / TCF-3 Antibody (aa429-581) LS-C132609
Sheep Polyclonal (IgG) to Human TCF7L1 / TCF-3
Human
Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human TCF7L1 / TCF-3
Human, Mouse, Rat, Bovine, Guinea pig, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human TCF7L1 / TCF-3
Rabbit
Human, Mouse, Rat, Bovine, Guinea pig, Rabbit (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

TCF7L1 / TCF-3 antibody was raised against synthetic peptide located between aa35-84 of human TCF7L1 (Q9HCS4, NP_112573). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Galago, Marmoset, Mouse, Rat, Bovine, Guinea pig (100%); Monkey, Elephant, Opossum (92%); Rabbit (85%).
LS-E10477 - Lyophilized - 100 µg - $145.00
aa35-84
Human TCF7L1
Immunohistochemistry: LS-B3476 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3476 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About TCF7L1 / TCF-3

Q9HCS4 NM_031283 NP_112573.1

TCF7L1 Antibody, TCF-3 Antibody, Transcription factor 7-like 1 Antibody, HMG box transcription factor 3 Antibody

Participates in the Wnt signaling pathway. Binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. Necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis (By similarity). Down-regulates NQO1, leading to increased mitomycin c resistance.

Immunohistochemistry

Immunohistochemistry
Anti-TCF7L1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3476 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-TCF7L1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3476 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
TCF7L1 antibody LS-B3476 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/7/2016

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