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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TCF7 Antibody (clone 2E9) LS‑C133471
TCF7 antibody LS-C133471 is an unconjugated mouse monoclonal antibody to human TCF7. Validated for ELISA and WB.
Catalog
Size
Price
LS-C133471-100
100 µg (0.5 mg/ml)
Unavailable

Popular TCF7 Products

Anti-TCF7 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1003 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bat, Bovine, Guinea pig, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, ICC, Western blot, ELISA
Antibody
Species: Human, Mouse, Rat
Applications: Western blot, Immunoprecipitation
Antibody
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Immunohistochemical analysis of TCF7 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Bovine
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation

Product Description

TCF7 antibody LS-C133471 is an unconjugated mouse monoclonal antibody to human TCF7. Validated for ELISA and WB.
About TCF7
Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4+ CD8+ immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1. P36402 NM_003202 NP_003193.2

TCF7 Antibody, TCF-7 Antibody, TCF1 Antibody, Transcription factor 7 Antibody, T-cell factor 1 Antibody, TCF-1 Antibody


Specifications

Target
Human TCF7
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal [2E9]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Failed Applications
  • IHC - Paraffin
Immunogen
TCF7 antibody was raised against tCF7 (NP_003193, 287 a.a. ~ 385 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human TCF7
Usage
Sandwich ELISA: Recombinant protein. Western Blot using cell lysates and the recombinant protein used as the immunogen.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Western blot

TCF7 monoclonal antibody (M02), clone 2E9 Western blot of TCF7 expression in Jurkat.
TCF7 monoclonal antibody (M02), clone 2E9 Western blot of TCF7 expression in Jurkat.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged TCF7 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged TCF7 is approximately 0.03 ng/ml as a capture antibody.

Western blot

TCF7 monoclonal antibody (M02), clone 2E9 Western blot of TCF7 expression in Jurkat.
TCF7 monoclonal antibody (M02), clone 2E9 Western blot of TCF7 expression in Jurkat.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged TCF7 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged TCF7 is approximately 0.03 ng/ml as a capture antibody.

Western blot

TCF7 monoclonal antibody (M02), clone 2E9 Western blot of TCF7 expression in Jurkat.
TCF7 monoclonal antibody (M02), clone 2E9 Western blot of TCF7 expression in Jurkat.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged TCF7 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged TCF7 is approximately 0.03 ng/ml as a capture antibody.

Western blot

TCF7 monoclonal antibody (M02), clone 2E9 Western blot of TCF7 expression in Jurkat.
TCF7 monoclonal antibody (M02), clone 2E9 Western blot of TCF7 expression in Jurkat.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged TCF7 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged TCF7 is approximately 0.03 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/21/2018

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