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Anti-TCF7 Antibody (aa228-277) IHC-plus™ LS-B1003

Note: This antibody replaces LS-C29952

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TCF7 Antibodies

Anti-TCF7 Antibody (aa228-277) IHC-plus™ LS-B1003
Rabbit Polyclonal to Human TCF7
Human, Monkey, Bat, Bovine, Guinea pig, Horse, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-TCF7 Antibody (N-Terminus, PE) LS-C283677
Rabbit Polyclonal (IgG) to Human TCF7
Human, Mouse
Western blot, ELISA
PE Conjugated
Anti-TCF7 Antibody (N-Terminus) LS-C352911
Rabbit Polyclonal to Human TCF7
Human, Bovine
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human TCF7
Human, Monkey, Bat, Bovine, Guinea pig, Horse, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human TCF7
Rabbit
Human, Monkey, Bat, Bovine, Guinea pig, Horse, Rabbit (tested or 100% immunogen sequence identity)
Mouse, Rat, Dog, Pig (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.13 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

TCF7 antibody was raised against synthetic peptide located between aa228-277 of human TCF7 (Q86WR9, NP_003193). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Bat, Horse, Guinea pig (100%); Galago, Mouse, Rat, Panda, Dog, Pig (92%); Elephant, Bovine, Rabbit (85%).
LS-E12393 - Lyophilized - 100 µg - $145.00
aa228-277
Human TCF7
Immunohistochemistry: LS-B1003 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1003 was determined to be 5 ug/ul.Antibody has been tested in peptide-based ELISA.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl After adding water, will consist of PBS buffer with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About TCF7

P36402 NM_003202 NP_003193.2

TCF7 Antibody, TCF-7 Antibody, TCF1 Antibody, Transcription factor 7 Antibody, T-cell factor 1 Antibody, TCF-1 Antibody

Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4+ CD8+ immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.

Immunohistochemistry

Immunohistochemistry
Anti-TCF7 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1003 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
TCF7 antibody LS-B1003 Western blot of Fetal thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Fetal Thymus Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/5/2016

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