LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
TBX1 / Brachyury Antibody (aa396‑445) LS‑C31179 Best Seller
Brachyury antibody LS-C31179 is an unconjugated rabbit polyclonal antibody to Brachyury (TBX1) from human, mouse, rat and other species. Validated for IHC and WB. Cited in 1 publication.
Catalog
Size
Price
LS-C31179-100
100 µg
$325

Popular TBX1 / Brachyury Products

Anti-TBX1 / Brachyury antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10717 dilution 1:75.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Lane: Fetal Lung Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Guinea pig, Rabbit
Applications: Western blot
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Mouse
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Immunohistochemical analysis of TBX1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot

Product Description

Brachyury antibody LS-C31179 is an unconjugated rabbit polyclonal antibody to Brachyury (TBX1) from human, mouse, rat and other species. Validated for IHC and WB. Cited in 1 publication.

Specifications

Target
Human TBX1 / Brachyury
Synonyms
TBX1, Brachyury, DGCR, DORV, DGS, T-box 1, T-box 1 transcription factor C, TBX1C, TGA, CAFS, CTHM, T-box protein 1, Testis-specific T-box protein, VCFS
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Guinea pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (5 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa396-445 of human TBX1 (O43435-3, NP_542378). Percent identity by BLAST analysis: Human, Gibbon, Mouse, Rat, Elephant, Dog, Rabbit, Guinea pig, Zebra finch (100%); Galago, Pig (92%).
Blocking Peptide
LS-E11788 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C31179. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa396-445
Specificity
Human TBX1
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Short term store at 2°C to 8°C for up to 1 week. For long term storage aliquot and store at -20°C.
Restrictions
For research use only.
About TBX1 / Brachyury
TBX1 / Brachyury is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product shares 98% amino acid sequence identity with the mouse ortholog. O43435 NM_005992 NP_005983.1


Publications (1)

1
Rebalancing gene haploinsufficiency in vivo by targeting chromatin. Fulcoli FG, Franzese M, Liu X, Zhang Z, Angelini C, Baldini A. Nature communications. 2016 7:11688. (ChIP-Seq; Mouse) [Full Text Article] [PubMed:27256596] [PMC:PMC4895808]

Customer Reviews (0)


Images

Immunohistochemistry

TBX1 antibody LS-C31179 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TBX1 antibody LS-C31179 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

TBX1 antibody LS-C31179 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TBX1 antibody LS-C31179 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

TBX1 antibody LS-C31179 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TBX1 antibody LS-C31179 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

TBX1 antibody LS-C31179 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TBX1 antibody LS-C31179 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 10/23/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy