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Anti-TBCK / TBCKL Antibody (aa457-694) LS-C186227

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.41 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TBCK / TBCKL Antibodies

Anti-TBCK / TBCKL Antibody (aa287-336) LS-C108048
Rabbit Polyclonal (IgG) to Human TBCK / TBCKL
Human, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Western blot
Unconjugated
Western blot Image
Anti-TBCK / TBCKL Antibody (aa108-135, Biotin) LS-C241592
Rabbit Polyclonal (IgG) to Human TBCK / TBCKL
Human, Mouse
Western blot, ELISA
Biotin Conjugated
Anti-TBCK / TBCKL Antibody (aa108-135, PE) LS-C241595
Rabbit Polyclonal (IgG) to Human TBCK / TBCKL
Human, Mouse
Western blot, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human TBCK / TBCKL
Human, Chicken
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human TBCK / TBCKL
Rabbit
Human, Chicken (tested or 100% immunogen sequence identity)
Rat, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

TBCK / TBCKL antibody was raised against recombinant fragment corresponding to a region within amino acids 457 and 694 of TBCK (SwissProt Q8TEA7). Percent identity by BLAST analysis: Human, Chicken (100%); Rat (99%); Xenopus, Zebrafish (98%).
aa457-694
Human TBCK
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About TBCK / TBCKL

Q8TEA7 NM_033115 NP_149106.2

TBCK Antibody, TBCKL Antibody, TBC1 domain containing kinase Antibody, HSPC302 Antibody

TBCK / TBCKL encodes a protein that contains a protein kinase domain, a Rhodanase-like domain and the Tre-2/Bub2/Cdc16 (TBC) domain. The encoded protein is thought to play a role in actin organization, cell growth and cell proliferation by regulating the mammalian target of the rapamycin (mTOR) signaling pathway. This protein may also be involved in the transcriptional regulation of the components of the mTOR complex. Alternative splicing results in multiple transcript variants.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded MCF7 xenograft using TBCK antibody at 1:100 dilution.

Immunocytochemistry

Immunocytochemistry
Immunofluorescence of paraformaldehyde-fixed HeLa using TBCK antibody at 1:200 dilution.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: NIH-3T3, B: JC, C: BCL-1. 7.5% SDS PAGE. TBCK antibody diluted at 1:1000.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: Hep G2 , B: Molt-4 . 7.5% SDS PAGE. TBCK antibody diluted at 1:1000.

Requested From: United States
Date Requested: 12/9/2016

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