Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IHC - Paraffin
IHC - Frozen (1:400 - 1:800)
Specificity and Use
Substance P conjugated to BSA.
Recognizes Substance P. Absorption with 10-100 ug SP and NKA per ml diluted antiserum abolishes the staining while Neurokinin A does not. Species cross-reactivity: Human, porcine, rat, mouse.
Suitable for use in Immunofluorescence and Immunohistochemistry. Immunohistochemistry (paraffin and frozen sections): 1:400-1:800 using immunofluorescence microscopy, with overnight incubation at 4?C. Positive control: Frozen sections of rat colon.
Lyophilized powder. Reconstitute, 50-100 ul ddH2O or 40-50% glycerol for long-term storage. Dilute further in 0.1 M PBS, 1% BSA, 0.1% sodium azide.
50 µl Reconstitute with 100ul sterile ddH2O or sterile 40-50% glycerol.
TAC1 / Substance P is four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Substance P is an antimicrobial peptide with antibacterial and antifungal properties.