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Anti-SUPT16H / FACTP140 Antibody (clone 8D2) IHC-plus™ LS-B1906

Note: This antibody replaces LS-C41103

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SUPT16H / FACTP140 Antibodies

Anti-SUPT16H / FACTP140 Antibody (aa321-640) IHC-plus™ LS-B753
Rabbit Polyclonal to Human SUPT16H / FACTP140
Human, Mouse
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-SUPT16H / FACTP140 Antibody (clone 8D2) IHC-plus™ LS-B1906
Mouse Monoclonal [clone 8D2] (IgG2a) to Human SUPT16H / FACTP140
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SUPT16H / FACTP140 Antibody (aa385-397) IHC-plus™ LS-B4454
Goat Polyclonal to Human SUPT16H / FACTP140
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Hamster, Pig
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (2)
Mouse Monoclonal [clone 8D2] (IgG2a) to Human SUPT16H / FACTP140
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human SUPT16H / FACTP140
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [8D2]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot

Specificity and Use

SUPT16H / FACTP140 antibody was raised against recombinant human SUPT16H (full length).
Human, weakly reacts with mouse Spt16
Immunohistochemistry: LS-B1906 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1906 was determined to be 10 ug/ml.

Packaging

Phosphate-buffered solution, pH 7.2, 0.09% sodium azide. The.
Store at 4°C. Do not freeze.
For research use only.

Publications (2)

1
DNA ends alter the molecular composition and localization of Ku multicomponent complexes. Adelmant G, Calkins AS, Garg BK, Card JD, Askenazi M, Miron A, Sobhian B, Zhang Y, Nakatani Y, Silver PA, Iglehart JD, Marto JA, Lazaro JB. Molecular & cellular proteomics : MCP. 2012 11:411-21. [PubMed:22535209] [PMC:PMC3412971]
2
Myogenin Recruits the Histone Chaperone Facilitates Chromatin Transcription (FACT) to Promote Nucleosome Disassembly at Muscle-specific Genes. Lolis AA, Londhe P, Beggs BC, Byrum SD, Tackett AJ, Davie JK. The Journal of biological chemistry. 2013 288:7676-87. [PubMed:23364797] [PMC:PMC3597808]

About SUPT16H / FACTP140

Q9Y5B9 NM_007192 NP_009123.1

SUPT16H Antibody, CDC68 Antibody, FACT140 Antibody, FACT Antibody, FACT complex subunit SPT16 Antibody, FACT 140 kDa subunit Antibody, HSPT16 Antibody, SPT16/CDC68 Antibody, FACTP140 Antibody

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure.

Immunohistochemistry

Immunohistochemistry
Anti-SUPT16H antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1906 concentration 10 ug/ml.

Western blot

Western blot
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Spt16 (Clone 8D2) antibody. Proteins were visualized using a donkey anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.

Requested From: United States
Date Requested: 12/2/2016

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