Target
Human SULF2 / Sulfatase 2
Synonyms
SULF2 | Extracellular sulfatase Sulf-2 | KIAA1247 | Sulfatase 2 | HSULF-2 | SULF-2
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-DGRVYHVGLGDAAQPR, from the internal region of the protein sequence according to NP_061325.1NP_940998.1.
Specificity
Human SULF2 / Sulfatase 2. This antibody is expected to recognize both reported isoforms (NP_061325.1; NP_940998.1)
Applications
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 90kDa band observed in Human Ovary lysates (calculated MW of 98.2kDa according to NP_940998.1). Recommended concentration: 0.3-1 ug/ml. An additional band of 50kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SULF2 / Sulfatase 2