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Anti-STK11 / LKB1 Antibody (aa14-44) LS-C100773

Catalog Size Price
LS-C100773-400 400 µl Unavailable

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141 STK11 / LKB1 Antibodies

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Anti-STK11 / LKB1 Antibody (N-Terminus) IHC-plus™ LS-B592
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IHC - Paraffin, Western blot
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Immunohistochemistry Image
Anti-STK11 / LKB1 Antibody (aa14-44) LS-C100773
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Anti-STK11 / LKB1 Antibody (clone 27D10) IHC-plus™ LS-B6669
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Anti-STK11 / LKB1 Antibody (aa1-236, clone 10A3) LS-C336598
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Anti-STK11 / LKB1 Antibody (aa421-433) LS-C388827
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100% Guaranteed
Rabbit Polyclonal to Human STK11 / LKB1
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human STK11 / LKB1
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Rat, Chicken, Xenopus (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

  • IHC - Paraffin

Specificity and Use

LS-E8791 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C100773. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa14-44
This STK11 (LKB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-44 amino acids from the N-terminal region of human STK11 (LKB1).

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About STK11 / LKB1

Q15831 NM_000455 NP_000446.1

STK11 Antibody, HLKB1 Antibody, Peutz-Jeghers syndrome Antibody, PJS Antibody, Serine/threonine kinase 11 Antibody, Liver kinase B1 Antibody, LKB1 Antibody

Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of STK11 (LKB1) Antibody (N-term I29) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot
STK11 Antibody (I29) western blot of T47D cell line lysates (35 ug/lane). The STK11 antibody detected the STK11 protein (arrow).

Western blot

Western blot
Western blot of STK11 (arrow) using rabbit polyclonal STK11 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the STK11 gene (Lane 2) (Origene Technologies).

Flow Cytometry

Flow Cytometry
STK11 (LKB1) Antibody (N-term I29) flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: 
Date Requested: 6/22/2017

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