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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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STK11 / LKB1 Antibody (aa1‑433) LS‑C16228
LKB1 antibody LS-C16228 is an unconjugated mouse monoclonal antibody to LKB1 (STK11) from human, mouse and rat. Validated for ICC and WB.
Antibody
Catalog
Size
Price
LS-C16228-100
100 µg
$650

Popular STK11 / LKB1 Products

Anti-STK11 / LKB1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B592 concentration 5 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Anti-STK11 / LKB1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6669 dilution 1:50.
Species: Human, Monkey, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Human pancreas tissue using anti-STK11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using LKB1 Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

LKB1 antibody LS-C16228 is an unconjugated mouse monoclonal antibody to LKB1 (STK11) from human, mouse and rat. Validated for ICC and WB.
About STK11 / LKB1
Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Q15831 NM_000455 NP_000446.1

STK11 Antibody, HLKB1 Antibody, Peutz-Jeghers syndrome Antibody, PJS Antibody, Serine/threonine kinase 11 Antibody, Liver kinase B1 Antibody, LKB1 Antibody


Specifications

Target
Human STK11 / LKB1
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • ICC (5 µg/ml)
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
STK11 / LKB1 antibody was raised against gST fusion protein corresponding to residues 1-433 of human LKB1.
Epitope
aa1-433
Specificity
Recognizes LKB1, Mr 51kD. An additional protein was also detected, Mr 90kD. Species cross-reactivity: Human, mouse and rat.
Usage
Suitable for use in Western Blot and Immunocytochemistry. Proteins were visualized using a IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Immunocytochemistry: 5 ug/ml detected LKB1 in transfected Cos-7 cells fixed with 3-7% paraformaldehyde and permeabilized with 0.1% Triton X-100.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 8/19/2018

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