Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- Western blot (1 - 3 µg/ml)
- ELISA (0.1 - 1 µg/ml)
- (applications tested for the base form of this product only)
STAT5B antibody was raised against a peptide corresponding to the unique carboxy-terminus of murine STAT5b coupled to a protein carrier.
Reacts specifically with the STAT 5b protein and does not exhibit any cross-reactivity with the highly related STAT 5a protein. Cross-reactivity with other endogenous STAT isoforms has not been observed. Species cross-reactivity: Human, mouse and rat. Reactivity of this antibody with STAT5b has been confirmed by Western blotting using cell lysates derived from human A431 cells (+/-EGF treatment), NIH 3T3 cells, MCF-7 cells and HeLa cells.
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Electrophoretic Mobility Shift Assay (EMSA). ELISA: 0.1-1 ug/ml. Immunoprecipitation: 5 ug. Western Blot: 1-3 ug/ml. Electrophoretic Mobility Shift Assay (EMSA): 1-3ug.
PBS, pH 7.4, 0.1% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.