Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
STAT5B antibody was raised against a peptide corresponding to the unique carboxy-terminus of murine STAT5b coupled to a protein carrier.
Reacts specifically with the STAT 5b protein and does not exhibit any cross-reactivity with the highly related STAT 5a protein. Cross-reactivity with other endogenous STAT isoforms has not been observed. Species cross-reactivity: Human, mouse and rat. Reactivity of this antibody with STAT5b has been confirmed by Western blotting using cell lysates derived from human A431 cells (+/-EGF treatment), NIH 3T3 cells, MCF-7 cells and HeLa cells.
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Electrophoretic Mobility Shift Assay (EMSA). ELISA: 0.1-1 ug/ml. Immunoprecipitation: 5 ug. Western Blot: 1-3 ug/ml. Electrophoretic Mobility Shift Assay (EMSA): 1-3ug.
PBS, pH 7.4, 0.1% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
STAT5B is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones.