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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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STAT5B Antibody (C‑Terminus) LS‑C10425
STAT5B antibody LS-C10425 is an unconjugated rabbit polyclonal antibody to STAT5B from human, mouse and rat. Validated for ELISA, GS, IP and WB.
Antibody
Catalog
Size
Price
LS-C10425-100
100 µg
$635

Popular STAT5B Products

Anti-STAT5B antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1679 dilution 1:50.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-STAT5B antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5540 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Anti-STAT5B antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4002 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Dog, Horse, Pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry of paraffin-embedded human placenta using antibody at 1:100 dilution.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA, Chromatin Immunoprecipitation
Antibody
Species:
Applications: Western blot, ELISA

Product Description

STAT5B antibody LS-C10425 is an unconjugated rabbit polyclonal antibody to STAT5B from human, mouse and rat. Validated for ELISA, GS, IP and WB.

Specifications

Target
Mouse STAT5B
Synonyms
STAT5B, Transcription factor STAT5B, STAT5
Host
Rabbit
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 - 3 µg/ml)
  • Immunoprecipitation
  • ELISA (0.1 - 1 µg/ml)
  • Gel shift
  • (applications tested for the base form of this product only)
Immunogen
STAT5B antibody was raised against peptide corresponding to the C-terminus of murine Stat5b coupled to protein carrier.
Epitope
C-Terminus
Specificity
Reacts specifically with the STAT5b protein and does not exhibit any cross-reactivity with the highly related STAT5a protein. Cross-reactivity with other endogenous STAT isoforms has not been observed. Specificity was confirmed by Western blot analysis using cell lysates derived from human A431 cells (+/-EGF treatment), NIH 3T3 cells, HeLa cells and MCF-7 cells. Species cross-reactivity: human, mouse and rat.
Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation. ELISA: 0.1-1 ug/ml. Immunoprecipitation: 5 ug. Western Blot: 1-3 ug/ml. Gel Mobility Shift Assay: 2.5-5 ug.
Presentation
PBS, pH 7.2, 0.1% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About STAT5B
STAT5B is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. P51692 NM_012448 NP_036580.2


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Requested From: United States
Date Requested: 10/16/2018

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