STAT5B antibody LS-C10425 is an unconjugated rabbit polyclonal antibody to STAT5B from human, mouse and rat. Validated for ELISA, GS, IP and WB.
Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- Western blot (1 - 3 µg/ml)
- ELISA (0.1 - 1 µg/ml)
- Gel shift
- (applications tested for the base form of this product only)
STAT5B antibody was raised against peptide corresponding to the C-terminus of murine Stat5b coupled to protein carrier.
Reacts specifically with the STAT5b protein and does not exhibit any cross-reactivity with the highly related STAT5a protein. Cross-reactivity with other endogenous STAT isoforms has not been observed. Specificity was confirmed by Western blot analysis using cell lysates derived from human A431 cells (+/-EGF treatment), NIH 3T3 cells, HeLa cells and MCF-7 cells. Species cross-reactivity: human, mouse and rat.
Suitable for use in ELISA, Western Blot, Immunoprecipitation. ELISA: 0.1-1 ug/ml. Immunoprecipitation: 5 ug. Western Blot: 1-3 ug/ml. Gel Mobility Shift Assay: 2.5-5 ug.
PBS, pH 7.2, 0.1% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.