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Anti-STAT5B Antibody (C-Terminus) LS-C10425

Ordering

Wt. Vol. Conc. Price
100 µg - - $495
Inquire for larger quantities

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Rabbit Polyclonal (IgG) to Mouse STAT5B
Mouse, Human, Rat
Western blot, Immunoprecipitation, ELISA, Gel shift
Unconjugated

Details

Mouse STAT5B
Rabbit
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

Western blot (1 - 3 µg/ml), Immunoprecipitation, ELISA (0.1 - 1 µg/ml), Gel shift

Specificity and Use

STAT5B antibody was raised against peptide corresponding to the C-terminus of murine Stat5b coupled to protein carrier.
C-Terminus
Reacts specifically with the STAT5b protein and does not exhibit any cross-reactivity with the highly related STAT5a protein. Cross-reactivity with other endogenous STAT isoforms has not been observed. Specificity was confirmed by Western blot analysis using cell lysates derived from human A431 cells (+/-EGF treatment), NIH 3T3 cells, HeLa cells and MCF-7 cells. Species cross-reactivity: human, mouse and rat.
Suitable for use in ELISA, Western Blot, Immunoprecipitation. ELISA: 0.1-1 ug/ml. Immunoprecipitation: 5 ug. Western Blot: 1-3 ug/ml. Gel Mobility Shift Assay: 2.5-5 ug.

Packaging

PBS, pH 7.2, 0.1% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About STAT5B

P51692 NM_012448 NP_036580.2

STAT5B Antibody, Transcription factor STAT5B Antibody, STAT5 Antibody

STAT5B is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones.

Requested From: United States
Date Requested: 10/1/2016

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