STAT5B antibody LS-C10427 is an unconjugated rabbit polyclonal antibody to STAT5B from human, mouse, rat and other species. Validated for IP and WB.
Human, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig
(tested or 100% immunogen sequence identity)
Bat, Chicken, Xenopus (at least 90% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot
- (applications tested for the base form of this product only)
STAT5B antibody was raised against amino acids 774-787 of human STAT5B (GRPMDSQWIPHAQS). The peptide was coupled to KLH prior to immunization. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig (100%); Panda, Bat, Chicken, Xenopus (93%); Opossum (86%).
Recognizes the 90-92kD forms of STAT5B protein. Species cross-reactivity: Human and mouse.
Western Blot: 0.5-2 mg/ml detects STAT5B (doublet of 90kD and 92kD) in. human A431 and mouse 3T3 fibroblasts using enhanced chemiluminescence with a five minute exposure. Immunoprecipitation: 4 ug immunoprecipitates STAT5B from A431 carcinoma cells and a. previous lot immunoprecipitated STAT5B from human Raji cells. A RIPA lysate of human A431 cells (500 ug protein) was incubated with 4 ug of anti-STAT 5B Immune complexes were precipitated with protein A agarose and analyzed by immunoblotting with anti- STAT 5B. Bands were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP in conjunction with enhanced chemiluminescence detection. Positive Antigen Control: mouse 3T3 cell lysate. Add 2.5 ul of 2-mercaptoethanol. per 100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels.
Supplied as a liquid in 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.