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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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STAT5B Antibody (aa774‑787) LS‑C10427
STAT5B antibody LS-C10427 is an unconjugated rabbit polyclonal antibody to STAT5B from human, mouse, rat and other species. Validated for IP and WB.
Antibody
Catalog
Size
Price
LS-C10427-200
200 µg
$605

Popular STAT5B Products

Anti-STAT5B antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1679 dilution 1:50.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-STAT5B antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5540 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Anti-STAT5B antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4002 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Dog, Horse, Pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry of paraffin-embedded human placenta using antibody at 1:100 dilution.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA, Chromatin Immunoprecipitation
Anti-STAT5B antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5541 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA

Product Description

STAT5B antibody LS-C10427 is an unconjugated rabbit polyclonal antibody to STAT5B from human, mouse, rat and other species. Validated for IP and WB.
About STAT5B
STAT5B is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. P51692 NM_012448 NP_036580.2

STAT5B Antibody, Transcription factor STAT5B Antibody, STAT5 Antibody


Specifications

Target
Human STAT5B
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)
Predicted
Bat, Chicken, Xenopus (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
STAT5B antibody was raised against amino acids 774-787 of human STAT5B (GRPMDSQWIPHAQS). The peptide was coupled to KLH prior to immunization. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig (100%); Panda, Bat, Chicken, Xenopus (93%); Opossum (86%).
Epitope
aa774-787
Specificity
Recognizes the 90-92kD forms of STAT5B protein. Species cross-reactivity: Human and mouse.
Usage
Western Blot: 0.5-2 mg/ml detects STAT5B (doublet of 90kD and 92kD) in. human A431 and mouse 3T3 fibroblasts using enhanced chemiluminescence with a five minute exposure. Immunoprecipitation: 4 ug immunoprecipitates STAT5B from A431 carcinoma cells and a. previous lot immunoprecipitated STAT5B from human Raji cells. A RIPA lysate of human A431 cells (500 ug protein) was incubated with 4 ug of anti-STAT 5B Immune complexes were precipitated with protein A agarose and analyzed by immunoblotting with anti- STAT 5B. Bands were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP in conjunction with enhanced chemiluminescence detection. Positive Antigen Control: mouse 3T3 cell lysate. Add 2.5 ul of 2-mercaptoethanol. per 100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels.
Presentation
Supplied as a liquid in 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.

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Requested From: United States
Date Requested: 9/20/2018

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