Human, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig
(tested or 100% immunogen sequence identity)
Bat, Chicken, Xenopus (at least 90% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot
- (applications tested for the base form of this product only)
STAT5B antibody was raised against amino acids 774-787 of human STAT5B (GRPMDSQWIPHAQS). The peptide was coupled to KLH prior to immunization. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig (100%); Panda, Bat, Chicken, Xenopus (93%); Opossum (86%).
Recognizes the 90-92kD forms of STAT5B protein. Species cross-reactivity: Human and mouse.
Western Blot: 0.5-2 mg/ml detects STAT5B (doublet of 90kD and 92kD) in. human A431 and mouse 3T3 fibroblasts using enhanced chemiluminescence with a five minute exposure. Immunoprecipitation: 4 ug immunoprecipitates STAT5B from A431 carcinoma cells and a. previous lot immunoprecipitated STAT5B from human Raji cells. A RIPA lysate of human A431 cells (500 ug protein) was incubated with 4 ug of anti-STAT 5B Immune complexes were precipitated with protein A agarose and analyzed by immunoblotting with anti- STAT 5B. Bands were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP in conjunction with enhanced chemiluminescence detection. Positive Antigen Control: mouse 3T3 cell lysate. Add 2.5 ul of 2-mercaptoethanol. per 100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels.
Supplied as a liquid in 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.