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Anti-STAT5B Antibody (aa774-787) LS-C10427

Ordering

Wt. Vol. Conc. Price
200 µg - - $530
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular STAT5B Antibodies

Anti-STAT5B Antibody (N-Terminus) IHC-plus™ LS-B1679
Rabbit Polyclonal (IgG) to Human STAT5B
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-STAT5B Antibody (N-Terminus) IHC-plus™ LS-B4002
Rabbit Polyclonal (IgG) to Human STAT5B
Human, Mouse, Rat, Dog, Horse, Pig, Rabbit, Chicken
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-STAT5B Antibody (clone 5D6) IHC-plus™ LS-B5540
Mouse Monoclonal [clone 5D6] (IgG1,k) to Human STAT5B
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human STAT5B
Human, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig
Western blot, Immunoprecipitation
Unconjugated

Details

Human STAT5B
Rabbit
Human, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)
Bat, Chicken, Xenopus (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot
  • Immunoprecipitation

Specificity and Use

STAT5B antibody was raised against amino acids 774-787 of human STAT5B (GRPMDSQWIPHAQS). The peptide was coupled to KLH prior to immunization. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig (100%); Panda, Bat, Chicken, Xenopus (93%); Opossum (86%).
aa774-787
Recognizes the 90-92kD forms of STAT5B protein. Species cross-reactivity: Human and mouse.
Western Blot: 0.5-2 mg/ml detects STAT5B (doublet of 90kD and 92kD) in. human A431 and mouse 3T3 fibroblasts using enhanced chemiluminescence with a five minute exposure. Immunoprecipitation: 4 ug immunoprecipitates STAT5B from A431 carcinoma cells and a. previous lot immunoprecipitated STAT5B from human Raji cells. A RIPA lysate of human A431 cells (500 ug protein) was incubated with 4 ug of anti-STAT 5B Immune complexes were precipitated with protein A agarose and analyzed by immunoblotting with anti- STAT 5B. Bands were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP in conjunction with enhanced chemiluminescence detection. Positive Antigen Control: mouse 3T3 cell lysate. Add 2.5 ul of 2-mercaptoethanol. per 100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels.

Packaging

Supplied as a liquid in 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About STAT5B

P51692 NM_012448 NP_036580.2

STAT5B Antibody, Transcription factor STAT5B Antibody, STAT5 Antibody

STAT5B is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones.

Requested From: United States
Date Requested: 12/4/2016

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