Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
IHC - Paraffin (20 - 40 µg/ml)
Western blot (1:500 - 1:2000)
Specificity and Use
STAT5 A+B antibody was raised against synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5 alpha protein.
A BLAST analysis was used to suggest cross-reactivity with Stat5 alpha from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5 alpha from other sources has not been determined.
Immunohistochemistry: LS-B2431 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2431 was determined to be 20-40 ug/ml. Western Blot: Expect a band approximately 91 kD in size corresponding to phosphorylated Stat5a protein in the appropriate cell lysate or extract. This phosphospecific polyclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the nonphosphorylated form of the immunizing peptide.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
Anti-STAT5 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2431 concentration 40 ug/ml.
Anti-STAT5 pY694 Antibody - Western Blot. Western blot of affinity purified anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kD) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1000 dilution, overnight at 4C. Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.