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Anti-STAT5 A+B Antibody (phospho-Tyr694) IHC-plus™ LS-B2431

Note: This antibody replaces LS-C60124

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Related Products

Most Popular STAT5 A+B Antibodies

Anti-STAT5 A+B Antibody (phospho-Tyr694) LS-C39269
Rabbit Monoclonal (IgG) to Rat STAT5 A+B
Rat, Human, Mouse
IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
Anti-STAT5 A+B Antibody (C-Terminus) LS-C51708
Rabbit Polyclonal to Mouse STAT5 A+B
Mouse, Human, Rat
IHC, Western blot, Immunoprecipitation
Unconjugated
Anti-STAT5 A+B Antibody (phospho-Tyr694/Tyr699) LS-C354261
Rabbit Polyclonal to Human STAT5 A+B
Human, Mouse, Rat, Bovine, Pig, Sheep, Chicken
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

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Rabbit Polyclonal to Mouse STAT5 A+B
Mouse, Human, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Mouse STAT5 A+B
Rabbit
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (20 - 40 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:25000)

Specificity and Use

STAT5 A+B antibody was raised against synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5 alpha protein.
pTyr694
A BLAST analysis was used to suggest cross-reactivity with Stat5 alpha from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5 alpha from other sources has not been determined.
Immunohistochemistry: LS-B2431 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2431 was determined to be 20-40 ug/ml. Western Blot: Expect a band approximately 91 kD in size corresponding to phosphorylated Stat5a protein in the appropriate cell lysate or extract. This phosphospecific polyclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the nonphosphorylated form of the immunizing peptide.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About STAT5 A+B


Immunohistochemistry

Immunohistochemistry
Anti-STAT5 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2431 concentration 40 ug/ml.

Western blot

Western blot
Anti-STAT5 pY694 Antibody - Western Blot. Western blot of affinity purified anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kD) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1000 dilution, overnight at 4C. Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.

Requested From: United States
Date Requested: 12/3/2016

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