Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Western blot, Immunoprecipitation (1:1000)
Specificity and Use
STAT5 A+B antibody was raised against synthetic peptide (DQAP[pS]PAVC) corresponding to aa722-730 of human STAT5A or 727-735 of human STAT5B (KLH).
Recognizes human phosphorylated STAT5A (S726) and STAT5B (S731). Does not recognize STAT5B containing a S731A mutation in lysates of transfected Cos-1 cells, stimulated with prolactin. Species cross-reactivity: Mouse and rat. Species sequence homology: bovine and sheep.
Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Phosphospecificity was confirmed by dephosphorylation of the STAT5a protein with l-phosphatase.
0.1% sodium azide, 30% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.