Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
- Western blot
- Immunoprecipitation (1:1000)
STAT5 A+B antibody was raised against synthetic peptide (DQAP[pS]PAVC) corresponding to aa722-730 of human STAT5A or 727-735 of human STAT5B (KLH).
Recognizes human phosphorylated STAT5A (S726) and STAT5B (S731). Does not recognize STAT5B containing a S731A mutation in lysates of transfected Cos-1 cells, stimulated with prolactin. Species cross-reactivity: Mouse and rat. Species sequence homology: bovine and sheep.
Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Phosphospecificity was confirmed by dephosphorylation of the STAT5a protein with l-phosphatase.
0.1% sodium azide, 30% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.