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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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STAT5 A+B Antibody (aa66‑80) LS‑C10415
STAT5 A+B antibody LS-C10415 is an unconjugated rabbit polyclonal antibody to STAT5 A+B from human, mouse, rat and other species. Validated for IHC, IP and WB.
Catalog
Size
Price
LS-C10415-250
250 µl
$620
Description
STAT5 A+B antibody LS-C10415 is an unconjugated rabbit polyclonal antibody to STAT5 A+B from human, mouse, rat and other species. Validated for IHC, IP and WB.
Target
Human STAT5 A+B
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Sheep, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
STAT5 A+B antibody was raised against kLH conjugated synthetic peptide (C-QELQKKAEHQVGEDG), corresponding to amino acids 66-80 of Human STAT5 A&B, with the N-terminal cysteine having been added. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Xenopus, Zebrafish (100%); Chicken, Trout, Salmon (93%); Pufferfish (87%).
Epitope
aa66-80
Specificity
Recognizes human STAT5 A & B; cross-reactivity unknown, although the immunogenic sequence is identical in mouse, rat and sheep.
Usage
Antibody Supershift: An Independent laboratory showed that Anti-STAT5 (A&B) supershifted IL-2 induced complexes in both fresh and preactivated peripheral blood lymphocytes. This antibody also supershifted the complexes induced by IL-2, IL-7 and IL-15, but not those induced by IL-4 and IL-13 in fresh peripheral blood lymphocytes. The specificity of the reaction was confirmed by competition with the immunogenic peptide (Lin, et al., 1995) Experimental conditions must be optimized to achieve the best supershift banding pattern (see Lin, et al., 1993). Western Blot Analysis: Not recommended. Immunoprecipitation: Not recommended. Immunocytochemistry: Not tested. Immunohistochemistry: Not tested.
Presentation
Serum
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Requested From: United States
Date Requested: 11/19/2018
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