Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Sheep, Xenopus, Zebrafish
(tested or 100% immunogen sequence identity)
Chicken (at least 90% immunogen sequence identity)
- Western blot
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STAT5 A+B antibody was raised against kLH conjugated synthetic peptide (C-QELQKKAEHQVGEDG), corresponding to amino acids 66-80 of Human STAT5 A&B, with the N-terminal cysteine having been added. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Xenopus, Zebrafish (100%); Chicken, Trout, Salmon (93%); Pufferfish (87%).
Recognizes human STAT5 A & B; cross-reactivity unknown, although the immunogenic sequence is identical in mouse, rat and sheep.
Antibody Supershift: An Independent laboratory showed that Anti-STAT5 (A&B) supershifted IL-2 induced complexes in both fresh and preactivated peripheral blood lymphocytes. This antibody also supershifted the complexes induced by IL-2, IL-7 and IL-15, but not those induced by IL-4 and IL-13 in fresh peripheral blood lymphocytes. The specificity of the reaction was confirmed by competition with the immunogenic peptide (Lin, et al., 1995) Experimental conditions must be optimized to achieve the best supershift banding pattern (see Lin, et al., 1993). Western Blot Analysis: Not recommended. Immunoprecipitation: Not recommended. Immunocytochemistry: Not tested. Immunohistochemistry: Not tested.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
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