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Anti-STAT3 Antibody (phospho-Ser727) LS-C97723

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular STAT3 Antibodies

Anti-STAT3 Antibody (clone 7G3H4) IHC-plus™ LS-B4102
Mouse Monoclonal [clone 7G3H4] (IgG1) to Human STAT3
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-STAT3 Antibody (aa683-732) IHC-plus™ LS-B4693
Rabbit Polyclonal to Human STAT3
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-STAT3 Antibody (C-Terminus) IHC-plus™ LS-B9434
Rabbit Polyclonal to Mouse STAT3
Mouse, Human, Rat
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human STAT3
Human, Mouse
ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human STAT3
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • ICC
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

STAT3 antibody was raised against kLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S727 of human STAT3.
LS-E3803 - Lyophilized - 100 µg - $145.00
pSer727

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About STAT3

P40763 NM_003150 NP_003141.2

STAT3 Antibody, Acute-phase response factor Antibody, APRF Antibody, DNA-binding protein APRF Antibody, HIES Antibody

STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of Phospho-STAT3-S727 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of SY5Y cells stained with phospho-STAT3-S727 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated phospho-STAT3-S727 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho-STAT3-S727 immunosignal to the nucleus and cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000168610).

Western blot

Western blot
The phospho-STAT3-Ser727 antibody is used in Western blot to detect Ser727-phosphorylated STAT3 in HL-60 tissue lysates collected before (-) or after (+) stimulation obtained by IFN-a.

Western blot

Western blot
The phospho-STAT3-Ser727 antibody is used in Western blot to detect Ser727-phosphorylated STAT3 in mouse liver tissue lysates collected before (-) or after (+) stimulation obtained by IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute (La Jolla, CA).

Requested From: United States
Date Requested: 12/5/2016

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