Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells.
Immunofluorescence: Alpha Fodrin Antibody - Mixed neuron-glial cultures stained with rabbit antibody to Alpha Fodrin (green) and mouse polyclonal antibody to Nestin (red). The Alpha Fodrin antibody stains numerous axonal and dendritic profiles in these cultures, clearly revealing the submembranous cytoskeleton. Since Alpha Fodrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody.
Western Blot: Alpha Fodrin Antibody - Western blot of mouse sciatic nerve extract (lanes 1 and 2) and brain homogenate (3). A prominent band running at 240kDa represents the intact Alpha Fodrin heavy chain. More minor bands below this likely represent in vivo proteolytic fragments of Alpha Fodrin.