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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPN / CD43 Antibody LS‑C13404
CD43 antibody LS-C13404 is an unconjugated mouse monoclonal antibody to rat CD43 (SPN). Validated for Flow and IHC.
Antibody
Catalog
Size
Price
LS-C13404-250
250 µg
$465

Popular SPN / CD43 Products

Antibody
Species: Human
Applications: Western blot, ELISA
Anti-SPN / CD43 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3775 concentration 20 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Antibody
Species: Rat
Applications: Flow Cytometry
Staining of C57Bl/6 splenocytes with APC anti-mo/hu B220 (RA3-6B2) (LS-C107315) and.25 ug of Biotin anti-mouse CD43 antibody followed by SAv-PE. Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Flow Cytometry
Staining of C57Bl/6 splenocytes with.25 ug of Purified Rat IgM isotype control (open histogram) or.25 ug of Purified anti-mouse CD43 antibody (colored histogram) followed by PE anti-rat IgM (HIS40) (LS-C107152). Cells in the lymphocyte gate were used for analysis.
Species: Mouse
Applications: Western blot, Immunoprecipitation, Flow Cytometry

Product Description

CD43 antibody LS-C13404 is an unconjugated mouse monoclonal antibody to rat CD43 (SPN). Validated for Flow and IHC.

Specifications

Target
Rat SPN / CD43
Synonyms
SPN, GPL115, Leukosialin, Leukocyte sialoglycoprotein, Galactoglycoprotein, GALGP, Sialophorin, LSN, CD43, CD43 antigen
Host
Mouse
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Flow Cytometry (1:50 - 1:100)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SPN / CD43 antibody was raised against rat thymocyte membrane preparation.
Specificity
Recognizes a monomorphic determinant expressed on rat thymocytes, T lymphocytes (but not B lymphocytes), plasma cells, polymorphs, stem cells and brain. The CD43 antigen recognized is a heavily glycosylated glycoprotein of mw 95000 which has a high content of O-linked carbohydrate structures. On thymocytes this glycoprotein is the main target for binding of peanut lectin. This antibody is useful for distinguishing B and T lymphocytes and also for analysis of NK cells and stem cells.
Usage
Flow Cytometry: 1:50-1:100. Immunohistochemistry: Frozen, Paraffin.
Presentation
PBS, pH 7.2, 0.1% Sodium Azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About SPN / CD43
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). P16150 NM_003123 NP_003114.1


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Requested From: United States
Date Requested: 10/22/2018

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