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SPN / CD43 Antibody LS‑C13404
CD43 antibody LS-C13404 is an unconjugated mouse monoclonal antibody to rat CD43 (SPN). Validated for Flow and IHC.
250 µg

Popular SPN / CD43 Products

Species: Human
Applications: Western blot, ELISA
Anti-SPN / CD43 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3775 concentration 20 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Species: Rat
Applications: Flow Cytometry
Staining of C57Bl/6 splenocytes with APC anti-mo/hu B220 (RA3-6B2) (LS-C107315) and.25 ug of Biotin anti-mouse CD43 antibody followed by SAv-PE. Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Flow Cytometry
Staining of C57Bl/6 splenocytes with.25 ug of Purified Rat IgM isotype control (open histogram) or.25 ug of Purified anti-mouse CD43 antibody (colored histogram) followed by PE anti-rat IgM (HIS40) (LS-C107152). Cells in the lymphocyte gate were used for analysis.
Species: Mouse
Applications: Western blot, Immunoprecipitation, Flow Cytometry

Product Description

CD43 antibody LS-C13404 is an unconjugated mouse monoclonal antibody to rat CD43 (SPN). Validated for Flow and IHC.


Rat SPN / CD43
SPN, GPL115, Leukosialin, Leukocyte sialoglycoprotein, Galactoglycoprotein, GALGP, Sialophorin, LSN, CD43, CD43 antigen
Rat (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Unconjugated. Also available conjugated with AP, Biotin, FITC, HRP, PE.
Protein G purified
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Flow Cytometry (1:50 - 1:100)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
SPN / CD43 antibody was raised against rat thymocyte membrane preparation.
Recognizes a monomorphic determinant expressed on rat thymocytes, T lymphocytes (but not B lymphocytes), plasma cells, polymorphs, stem cells and brain. The CD43 antigen recognized is a heavily glycosylated glycoprotein of mw 95000 which has a high content of O-linked carbohydrate structures. On thymocytes this glycoprotein is the main target for binding of peanut lectin. This antibody is useful for distinguishing B and T lymphocytes and also for analysis of NK cells and stem cells.
Flow Cytometry: 1:50-1:100. Immunohistochemistry: Frozen, Paraffin.
PBS, pH 7.2, 0.1% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
About SPN / CD43
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). P16150 NM_003123 NP_003114.1

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Requested From: United States
Date Requested: 10/22/2018

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